Themes in RNA-protein recognition
Themes in RNA-protein recognition
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DOI：
10.1006/jmbi.1999.2991
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发表时间：
1999-10-22
影响因子：
5.6
通讯作者：
Draper, DE
中科院分区：
文献类型：
--
作者：
Draper, DE
原子分辨率结构：现在可用于20多种蛋白质与特定RNA的复合物。本综述探讨了这一套结构中出现的两个主要主题。“沟结合剂”类蛋白质将蛋白质结构（α-螺旋、3（10）-螺旋、β-带或不规则环）放置在RNA螺旋的沟中，识别碱基的特定序列和沟的份额或尺寸，其有时从正常的A-形式扭曲。第二类蛋白质使用β折叠表面来创建检查单链RNA碱基的口袋。这些蛋白质中的一些识别完全非结构化的RNA，而在其他RNA二级结构中，通过将碱基限制在适当的方向上来间接促进结合。热力学研究表明，结合特异性通常是几个因素的函数，包括碱基特异性氢键、非极性接触以及蛋白质和RNA结合表面的相互调节。RNA结合蛋白所使用的识别策略和结构框架与DNA结合蛋白所使用的识别策略和结构框架并没有明显的不同，这表明这两种核酸结合蛋白并不是独立进化的。(C)北京：科学出版社.
Atomic resolution structures:are now available for more than 20 complexes of proteins with specific RNAs. This review examines two main themes that appear in this set of structures. A "groove binder" class of proteins places a protein structure (alpha-helix, 3(10)-helix, beta-ribbon, or irregular loop) in the groove of an RNA helix, recognizing both the specific sequence of bases and the share or dimensions of the groove, which are sometimes distorted from-the normal A-form. A second class of proteins uses beta-sheet surfaces to create pockets that examine single-stranded RNA bases. Some of these proteins recognize completely unstructured RNA, and in others RNA secondary structure indirectly promotes binding by constraining bases in an appropriate orientation. Thermodynamic studies have shown that binding specificity is generally a function of several factors, including base-specific hydrogen bonds, non-polar contacts, and mutual accommodation of the protein and RNA-binding surfaces. The recognition strategies and structural frameworks used by RNA binding proteins are not exotically different from those employed by DNA-binding proteins, suggesting that the two kinds of nucleic acid-binding proteins have not evolved independently. (C) 1999 Academic Press.