Hippocampal plasticity requires postsynaptic ephrinBs
Hippocampal plasticity requires postsynaptic ephrinBs
复制标题
DOI：
10.1038/nn1164
复制
发表时间：
2004-01-01
影响因子：
25
通讯作者：
Klein, R
中科院分区：
文献类型：
--
作者：
Grunwald, IC;Korte, M;Klein, R
化学突触包含调节突触传递和可塑性的特殊突触前和突触后结构。 EphB 受体酪氨酸激酶是这一过程中的重要分子成分。此前，EphB 受体被证明在突触后发挥作用，而其跨膜配体 ephrinB 则被认为在突触前发挥作用。在这里，我们表明，在小鼠海马 CA1 神经元中，Eph/ephrin 系统以相反的方式使用：ephrinB 主要位于突触后，是突触可塑性所必需的。我们进一步证明，EphA4（一种候选受体）也与独立于其细胞质结构域的长期可塑性密切相关，这表明 ephrinB 是活跃的信号传导伴侣。这项工作提出了一个有趣的可能性，即根据突触的类型，Eph/ephrins 可以以相反的方式参与活动依赖性可塑性，而 ephrinB 位于突触前或突触后侧。
Chemical synapses contain specialized pre- and postsynaptic structures that regulate synaptic transmission and plasticity. EphB receptor tyrosine kinases are important molecular components in this process. Previously, EphB receptors were shown to act postsynaptically, whereas their transmembrane ligands, the ephrinBs, were presumed to act presynaptically. Here we show that in mouse hippocampal CA1 neurons, the Eph/ephrin system is used in an inverted manner: ephrinBs are predominantly localized postsynaptically and are required for synaptic plasticity. We further demonstrate that EphA4, a candidate receptor, is also critically involved in long-term plasticity independent of its cytoplasmic domain, suggesting that ephrinBs are the active signaling partner. This work raises the intriguing possibility that depending on the type of synapse, Eph/ephrins can be involved in activity-dependent plasticity in converse ways, with ephrinBs on the pre- or the postsynaptic side.