Analysis of cardiomyocyte movement in the developing murine heart.
Analysis of cardiomyocyte movement in the developing murine heart.
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分析小鼠心脏发育中的心肌细胞运动。
DOI：
10.1016/j.bbrc.2015.07.036
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Fukuda K
中科院分区：
文献类型：
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作者：
Hashimoto H;Yuasa S;Tabata H;Tohyama S;Seki T;Egashira T;Hayashiji N;Hattori F;Kusumoto D;Kunitomi A;Takei M;Kashimura S;Yozu G;Shimojima M;Motoda C;Muraoka N;Nakajima K;Sakaue-Sawano A;Miyawaki A;Fukuda K
几种类型的心脏前体细胞的精确组合控制着心脏器官的发生。心脏前体细胞在发育早期呈动态运动，形成复杂的心脏结构。然而，新组织的哺乳动物心脏内的心肌细胞运动仍不清楚。我们之前建立了小鼠心脏的活体时间推移成像方法，通过使用Fucci（基于荧光泛素化的细胞周期指示剂）系统来研究心肌细胞的行为，该系统可以有效地将活心肌细胞中的单个G1，S/G2/M和G1/S过渡期细胞核分别标记为红色，绿色和黄色。对Fucci绿色阳性心室肌细胞基因表达的整体分析证实，在G1/S、S、G2/M和M相转变中表达的细胞周期调控基因上调。有趣的是，通路分析显示，许多与细胞周期相关的基因在Fucci绿色阳性心室心肌细胞中显著上调，而只有少数与细胞运动相关的基因上调。延时成像显示，小鼠增殖的心肌细胞在心脏内没有表现出动态运动，而是在进入细胞周期后停留在原地。
The precise assemblage of several types of cardiac precursors controls heart organogenesis. The cardiac precursors show dynamic movement during early development and then form the complicated heart structure. However, cardiomyocyte movements inside the newly organized mammalian heart remain unclear. We previously established the method ofex vivotime-lapse imaging of the murine heart to study cardiomyocyte behavior by using the Fucci (fluorescent ubiquitination-based cell cycle indicator) system, which can effectively label individual G1, S/G2/M, and G1/S-transition phase nuclei in living cardiomyocytes as red, green, and yellow, respectively. Global analysis of gene expression in Fucci green positive ventricular cardiomyocytes confirmed that cell cycle regulatory genes expressed in G1/S, S, G2/M, and M phase transitions were upregulated. Interestingly, pathway analysis revealed that many genes related to the cell cycle were significantly upregulated in the Fucci green positive ventricular cardiomyocytes, while only a small number of genes related to cell motility were upregulated. Time-lapse imaging showed that murine proliferating cardiomyocytes did not exhibit dynamic movement inside the heart, but stayed on site after entering the cell cycle.