ACTIVATION OF MAP KINASE KINASE IS NECESSARY AND SUFFICIENT FOR PC12 DIFFERENTIATION AND FOR TRANSFORMATION OF NIH 3T3 CELLS
ACTIVATION OF MAP KINASE KINASE IS NECESSARY AND SUFFICIENT FOR PC12 DIFFERENTIATION AND FOR TRANSFORMATION OF NIH 3T3 CELLS
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DOI：
10.1016/0092-8674(94)90133-3
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发表时间：
1994-06-17
期刊：
影响因子：
64.5
通讯作者：
MARSHALL, CJ
中科院分区：
文献类型：
--
作者：
COWLEY, S;PATERSON, H;MARSHALL, CJ
MAP激酶途径被多种导致细胞增殖或分化的外部信号激活。然而，目前尚不清楚该通路的激活是否需要细胞反应，或者它是否只是信号转导的一个分支点。为了研究这些问题，我们生成了MAP激酶激酶1的组成激活和干扰突变体。激活的突变体刺激PC12细胞神经元分化，转化NIH 3T3细胞。干扰突变体抑制生长因子诱导的PC12分化、生长因子刺激的增殖，并逆转v-src和ras转化的细胞。因此，这些结果表明，根据细胞环境，激活MAP激酶激酶是细胞分化或增殖的必要和充分条件。
The MAP kinase pathway is activated by a wide variety of external signals leading to cell proliferation or differentiation. However, it is not clear whether activation of this pathway is required for cellular responses or whether it is only one branch point in signal transduction. To investigate these questions, we generated constitutively activated and interfering mutants of MAP kinase kinase 1. The activated mutants stimulated PC12 cell neuronal differentiation and transformed NIH 3T3 cells. The interfering mutants inhibited growth factor-induced PC12 differentiation, growth factor stimulation of proliferation, and reverted v-src- and ras-transformed cells. These results therefore show that, depending on cellular context, activation of MAP kinase kinase is necessary and sufficient for cell differentiation or proliferation.