Observations on the behaviour of second stage juveniles of Heterodera schachtii inside host roots.
Observations on the behaviour of second stage juveniles of Heterodera schachtii inside host roots.
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发表时间：
1986
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通讯作者：
U. Wyss;U. Zunke
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作者：
U. Wyss;U. Zunke
利用特制的观察箱和高分辨率视频增强对比显微镜，研究了油菜幼苗根内感染夏氏胞囊线虫（Heterodera schachtii）的第二阶段幼虫的行为。用这种技术，细胞内迁移的青少年的合胞体诱导位点附近或血管组织内，并从最初的合胞体喂养，可以直接观察到活的动物。分析了线虫摄食器内部和最初合胞体中结构的功能，这些结构以前仅用透射电子显微镜记录。从最初的合胞体的进食总是在一系列重复的循环中，每个循环由三个不同的阶段组成：i）通过连接到管心针腔的孔口的进食管从修饰的细胞质区域内连续抽取营养物。针芯从进食部位缩回，针芯在气孔腔内轻微移动，针芯尖端重新插入，通过耗尽来自两个腹下食管腺壶腹的扩张的瓣膜内的分泌液而停止。iii）从背食道腺壶腹释放分泌液，在修饰的细胞质区域内形成新的进食管。事件发生在这三个阶段内的线虫的摄食器和初始合胞体的描述和讨论。
Using special observation chambers and high resolution video-enhanced contrast microscopy, the behaviour of infective second stage juveniles of Heterodera schachtii was studied inside roots of Brassica napus seedlings. With this technique, intracellular migration of the juveniles to the site of syncytium induction near or within the vascular tissue and feeding from the initial syncytium could, for the first time, be directly observed on live animals. The function of structures inside the feeding apparatus of the nematode and in the initial syncytium, previously only recorded with the transmission electron microscope, was analysed. Feeding from the initial syncytium was invariably in a series of repeated cycles, each composed of three distinct phases : i) continuous withdrawal of nutrients from within a zone of modified cytoplasm through a feeding tube connected to the orifice of the stylet lumen. iil stylet retraction from the feeding site, gentle stylet movements within t e stomatal cavity and reinsertion of the stylet-tip, accompanicd by depletion of secretory fluids from within the expanded valve membranes of the two subventral oesophageal gland ampullae. iii) release of secretory fluids from the ampulla of the dorsal œsophageal gland, forming a new feeding tube inside the zone of modified cytoplasm. Events that occurred during these three phases within the feeding apparatus of the nematode and in the initial syncytium are described and discussed.