Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase
Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase
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DOI：
10.1126/science.1066869
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发表时间：
2001-12-07
期刊：
影响因子：
56.9
通讯作者：
Fischetti, VA
中科院分区：
文献类型：
--
作者：
Loeffler, JM;Nelson, D;Fischetti, VA
鼻咽携带是社区中肺炎链球菌的主要宿主。虽然消除这一水库将大大减少疾病的发生，但没有适当的干预措施可用于这一目的。我们发现，在接触后几秒钟，一种纯化的肺炎球菌噬菌体裂解酶（pk10）能够杀死15种常见的肺炎球菌血清型，包括高度耐青霉素的菌株。在体内，先前定殖的小鼠在单一酶处理后5小时显示不可检测的肺炎球菌滴度。这种酶对人口咽部正常存在的微生物几乎没有影响，并且在广泛暴露于这种酶后无法检测到耐Pal的肺炎球菌。
Nasopharyngeal carriage is the major reservoir for Streptococcus pneumoniae in the community. Although eliminating this reservoir would greatly reduce disease occurrence, no suitable intervention has been available for this purpose. We show here that seconds after contact, a purified pneumococcal bacteriophage lytic enzyme (Pal) is able to kill 15 common serotypes of pneumococci, including highly penicillin-resistant strains. In vivo, previously colonized mice revealed undetectable pneumococcal titers 5 hours after a single enzyme treatment. Pal enzyme had little or no effect on microorganisms normally found in the human oropharynx, and Pal-resistant pneumococci could not be detected after extensive exposure to the enzyme.