A new type of recording chamber with an easy-to-exchange microdrive array for chronic recordings in macaque monkeys
A new type of recording chamber with an easy-to-exchange microdrive array for chronic recordings in macaque monkeys
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DOI：
10.1152/jn.00508.2010
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发表时间：
2011-06-01
影响因子：
2.5
通讯作者：
Wegener, Detlef
中科院分区：
文献类型：
--
作者：
Galashan, F. Orlando;Rempel, Hanna C.;Wegener, Detlef
[10]李国忠，李国忠.一种新型的记录室，具有易于更换的微驱动器阵列，用于猕猴的慢性记录。J Neurophysiol 105：3092-3105，2011.首次发表于2011年3月30日; doi：10.1152/jn.00508.2010.-在猴子中，用长期植入的微电极进行长期记录时，记录单个单位甚至小的多单位簇的概率经常不断下降。该问题与可用装置的两个技术限制相关联：第一，对电极移动的限制，以及第二，缺乏定期容易地更换电极的可能性。允许调整记录位置和使用具有适当电极的新记录轨道可以避免这些问题，并且使慢性记录更类似于急性记录。在这里，我们描述了一种新型的植入物来解决这个问题。它由一个新型的记录室和一个可互换的多电极阵列组成，多电极阵列可以精确地安装在记录室中，并可逆地固定在记录室中，在一分钟内，它可以与另一个装有新电极的阵列交换。该阵列允许六个电极的双向移动距离高达12 mm。记录室可以实现颅内空间的密封隔离，从而实现持久的无菌条件，并将硬脑膜增厚降至最低限度，这一点已通过微生物和组织病理学分析证实。该装置设计简单，易于制造，成本低。功能已在初级和次级视觉皮层的三只猕猴超过15个月的时间内进行了测试。结果表明，即使在一年多后，单单位和多单位的反应，可以获得高发病率。
Galashan FO, Rempel HC, Meyer A, Gruber-Dujardin E, Kreiter AK, Wegener D. A new type of recording chamber with an easy-to-exchange microdrive array for chronic recordings in macaque monkeys. J Neurophysiol 105: 3092-3105, 2011. First published March 30, 2011; doi:10.1152/jn.00508.2010.-In monkeys, long-term recordings with chronically implanted microelectrodes frequently suffer from a continuously decreasing probability to record single units or even small multiunit clusters. This problem is associated with two technical limitations of the available devices: first, restrictions for electrode movement, and second, absent possibility to exchange electrodes easily on a regular basis. Permitting to adjust the recording site and to use new recording tracks with proper electrodes may avoid these problems and make chronic more similar to acute recordings. Here, we describe a novel type of implant tackling this issue. It consists of a new type of recording chamber combined with an exchangeable multielectrode array that precisely fits into it. The multielectrode array is reversibly fixed to the chamber, and within a minute it can be exchanged against another array equipped with new electrodes at the awake animal. The array allows for bidirectional movement of six electrodes for a distance of up to 12 mm. The recording chamber enables hermetical isolation of the intracranial space, resulting in long-lasting aseptic conditions and reducing dural thickening to a minimum, as confirmed by microbiological and histopathological analysis. The device has a simple design and is both easy to produce and low in cost. Functionality has been tested in primary and secondary visual cortex of three macaque monkeys over a period of up to 15 mo. The results show that even after more than a year, single and multiunit responses can be obtained with high incidence.