Identification of Enzymes from <i>Pseudogluconobacter saccharoketogenes</i> Producing <scp>d</scp>-Glucaric Acid from <scp>d</scp>-Glucose
Identification of Enzymes from <i>Pseudogluconobacter saccharoketogenes</i> Producing <scp>d</scp>-Glucaric Acid from <scp>d</scp>-Glucose
复制标题
从<scp>d</scp>-葡萄糖生产<scp>d</scp>-葡萄糖酸的<i>产酮假葡萄糖杆菌</i>酶的鉴定
DOI：
10.1093/bbb/zbab182
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Kiryu Takaaki
中科院分区：
文献类型：
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作者：
Ito Tetsuya;Masaki Hisaharu;Fujita Koki;Murakami Hiromi;Shizuma Motohiro;Kiso Taro;Kiryu Takaaki
2004年，美国能源部将d-葡萄糖酸列为12大生物基化学品之一和潜在的生物聚合物构建单元。在这项研究中，我们表明，假葡萄糖酸糖酮生成菌株可以从d-葡萄糖生产-葡萄糖酸，尽管由于副产物2-酮-d-葡萄糖酸的大量产生而产量较低。为了提高葡萄糖二酸产量，我们生成了Rh47-3，aP。 saccharoketogenesIFO14464 突变体，其从 d-葡萄糖酸和 d-葡萄糖产生葡萄糖二酸，产率分别为 81 mol% 和 53 mol%。此外，还纯化了涉及ind-葡萄糖酸生产的关键酶，即乙醇脱氢酶（Ps-ADH）、乙醛脱氢酶（Ps-ALDH）和葡萄糖酸2-脱氢酶（Ps-GADH），并评估了它们在ind-葡萄糖酸合成中的作用。 Ps-ADH 和 Ps-ALDH 催化 d-葡萄糖二酸的产生，这是由 d-葡萄糖酸和 d-葡萄糖醛酸途径介导的。相反，Ps-GADH 通过促进从 d-葡萄糖形成 2-酮-d-葡萄糖酸来抑制 d-葡萄糖酸的产生。
In 2004, the US Department of Energy listedd-glucaric acid as one of the top 12 bio-based chemicals and a potential biopolymer building block. In this study, we show thatPseudogluconobacter saccharoketogenesstrains can produced-glucaric acid fromd-glucose, although in low yield because of the generation of the byproduct 2-keto-d-gluconic acid in large quantities. To improved-glucaric acid yield, we generated Rh47-3, aP. saccharoketogenesIFO14464 mutant, which producedd-glucaric acid fromd-gluconic acid andd-glucose with 81 and 53 mol% yields, respectively. Furthermore, the key enzymes involved ind-glucaric acid production, alcohol dehydrogenase (Ps-ADH), aldehyde dehydrogenase (Ps-ALDH), and gluconate 2-dehydrogenase (Ps-GADH), were purified and their roles ind-glucaric acid synthesis were evaluated. Ps-ADH and Ps-ALDH catalyzedd-glucaric acid production, which was mediated byd-gluconic acid andd-glucuronic acid pathways. In contrast, Ps-GADH inhibitedd-glucaric acid production by promoting the formation of 2-keto-d-gluconic acid fromd-glucose.