Intracellular distribution of rubella virus nonstructural protein P150
Intracellular distribution of rubella virus nonstructural protein P150
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DOI：
10.1128/jvi.73.9.7805-7811.1999
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发表时间：
1999-09-01
影响因子：
5.4
通讯作者：
Kääriäinen, L
中科院分区：
文献类型：
--
作者：
Kujala, P;Ahola, T;Kääriäinen, L
针对风疹病毒（RUB）非结构多聚蛋白氨基末端片段制备的抗血清被用于研究风疹病毒感染的非洲绿猴肾细胞（Vero细胞）。复制酶蛋白P150与内溶酶体来源的囊泡和液泡相关联，随后与大型、卷曲的管状膜结构相关。新掺入的溴尿苷与相同结构相关，特别是与小的膜内陷结构（小球体）相关，这表明这些结构可能是病毒RNA合成的位点。
Antiserum prepared against an amino-terminal fragment of rubella virus (RUB) nonstructural polyprotein was used to study RUB-infected Vero cells. Replicase protein P150 was associated with vesicles and vacuoles of endolysosomal origin and later with large, convoluted, tubular membrane structures. Newly incorporated bromouridine was associated with the same structures and specifically with small membrane invaginations, spherules, indicating that these structures may he the sites of viral RNA synthesis.