Calcium gradients underlying cell migration
Calcium gradients underlying cell migration
复制标题
细胞迁移的钙梯度
DOI：
10.1016/j.ceb.2011.12.002
复制
发表时间：
2012-04-01
影响因子：
7.5
通讯作者：
Cheng, Heping
中科院分区：
文献类型：
--
作者：
Wei, Chaoliang;Wang, Xianhua;Cheng, Heping
钙离子是生物学中最简单、用途最广的第二信使。迁移细胞携带大量的钙效应蛋白，呈现出精细的多尺度、多层次的细胞内钙动力学结构。例如，在运动成纤维细胞中，存在直径约为5 μ m、持续时间为10-2000 ms的瞬态钙微域（“钙闪烁”），闪烁活动梯度沿前后轴上升，背景钙浓度梯度沿相反方向浅。当受到外部引导信号梯度的影响时，在前沿产生局部闪烁梯度，引导细胞转向新的方向，这是由闪烁活动的不对称性决定的，显然是一种随机决策机制。这些最近的发现让我们得以一窥时空协调的钙梯度是如何协调像定向运动一样复杂的细胞行为的。
The calcium ion is the simplest and most versatile second messenger in biology. Harboring a myriad of calcium effector proteins, migrating cells display an exquisite multiscaled and multilayered architecture of intracellular calcium dynamics. In motile fibroblasts, for instance, there are transient calcium microdomains ('calcium flickers') of similar to 5 mu m in diameter and 10-2000 ms in duration, a rising flicker activity gradient along the rear-to-front axis, and a shallow background calcium concentration gradient in the opposite direction. When subjected to external gradients of guidance cues, local flicker gradients are created de nova in the leading edge, which steer cells to turn in new directions as defined by the asymmetry of the flicker activity, apparently by a stochastic decision-making mechanism. These recent findings provide a glimpse into how spatiotemporally coordinated calcium gradients orchestrate cellular behavior as complex as directional movement.