2,3,7,8-Tetrachlorodibenzo-p-dioxin-induced cleft palate in the mouse: evidence for alterations in palatal shelf fusion.
2,3,7,8-Tetrachlorodibenzo-p-dioxin-induced cleft palate in the mouse: evidence for alterations in palatal shelf fusion.
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2,3,7,8-四氯二苯并-对二恶英诱导的小鼠腭裂：腭架融合改变的证据。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
V. Diewert
中科院分区：
文献类型：
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作者：
Robert M. Pratt;Lennart Dencker;V. Diewert
2，3，7，8-四氯二苯并-对-二恶英（TCDD）在包括C57 BL/6 J在内的某些品系小鼠的器官发生过程中给药时，可导致胎儿腭裂的比例很高，但在某些其他品系（AKR/J）中不会。本研究的目的是研究各种生物化学和形态学方面的TCDD引起的变化，在发展中的腭架。我们的研究结果表明，当TCDD（100微克/公斤），在个别天之间的第8和第10天的妊娠，腭裂的比例很高。在C57 BL/6 J中检测到TCDD特异性受体，但在AKR/J中未检测到。与包括肝脏在内的其他胚胎组织相比，第13天的腭架中TCDD受体的量最高。在腭部抬高和融合的时间从胚胎中取出的冷冻切片的检查表明，TCDD不干扰腭架的生长、抬高或初始接触，但干扰中膜上皮细胞的牢固粘附和/或变性。我们的研究结果表明，TCDD对胚胎腭架产生直接影响，导致腭裂的形成。
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) causes a high percentage of cleft palate in fetuses when administered during organogenesis in certain strains of mice including the C57BL/6J, but not in certain other strains (AKR/J). The purpose of the present study was to examine various biochemical and morphological aspects of TCDD-induced changes in the developing palatal shelves. Our results indicate that when TCDD (100 micrograms/kg) was given on individual days between days 8 and 10 of gestation, a high percentage of cleft palate was observed. Receptors specific for TCDD were detected in the C57BL/6J but not AKR/J palatal shelves. The amount of TCDD receptors is highest in the palatal shelves on day 13 as compared to other embryonic tissues including the liver. Examination of cryostat sections taken from embryos during the time of palatal elevation and fusion demonstrated that TCDD does not interfere with growth, elevation, or initial contact of the palatal shelves, but does interfere with firm adhesion and/or degeneration of the medial epithelial cells. Our results suggest that TCDD exerts a direct effect on the embryonic palatal shelves which results in formation of cleft palate.
2,3,7,8-四氯二苯并-对二恶英在 C57BL/6J、DBA/2J 和 B6D2F1/J 小鼠中的分布、排泄和代谢。
DOI：
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发表时间：
1983
期刊：
Drug metabolism and disposition: the biological fate of chemicals
影响因子：
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作者：
Gasiewicz,TA;Geiger,LE;Rucci,G;Neal,RA
通讯作者：
Neal,RA