Susceptibility and potential biochemical mechanism of Oedaleus asiaticus to beta-cypermethrin and deltamethrin in the Inner Mongolia, China
Susceptibility and potential biochemical mechanism of Oedaleus asiaticus to beta-cypermethrin and deltamethrin in the Inner Mongolia, China
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DOI：
10.1016/j.pestbp.2015.11.011
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发表时间：
2016-09-01
影响因子：
4.7
通讯作者：
Zhang, Jianzhen
中科院分区：
文献类型：
--
作者：
Dong, Wei;Zhang, Xubo;Zhang, Jianzhen
亚洲斑潜蝇(Oedaleus Asiaticus)是内蒙古地区一种极具破坏性的杂草害虫中国，由于频繁使用拟除虫菊酯类杀虫剂防治该蝗虫，可能对拟除虫菊酯产生抗药性。本文研究了亚洲玉米象5个田间种群对两种拟除虫菊酯类杀虫剂的敏感性。乌拉特中旗(WB)种群最不敏感，而鄂温克旗(EB)种群似乎最敏感。白纹伊蚊种群对高效氯氰菊酯和溴氰菊酯的敏感性分别是EB种群的3.16倍和5.15倍。此外，我们还测定了羧酸酯酶(CARE)和谷胱甘肽-S-转移酶(GST)的酶活性和基因表达水平，发现WB群体的活性分别是EB群体的5.15倍和2.8倍。实时定量聚合酶链式反应(qRT-PCR)分析表明，WB、四子王旗和EB人群中CARE和GST基因的mRNA表达水平与LD50呈正相关。我们的发现表明，亚洲稻瘟病菌对拟除虫菊酯的敏感性差异可能归因于CARE和GST基因活性和mRNA表达水平的提高。(C)2015 Elsevier Inc.保留所有权利。
Oedaleus asiaticus is a highly destructive grass pest in Inner Mongolia, China, and likely developed resistance to pyrethroid insecticides due to their frequent application for control of this locust. In this study, the susceptibility of five field populations of O. asiaticus to two pyrethroid insecticides was investigated. The Wulate Middle Banner (WB) population was the least susceptible, whereas the Ewenki Banner (EB) population appeared to be the most sensitive. The WB population was 3.16 and 5.15-fold less sensitive to beta-cypermethrin and deltamethrin than EB population, respectively. Further, the enzyme activities and mRNA expression levels of carboxylesterase (CarE) and glutathione-S-transferase (GST) were determined and we found that their activities in the WB population were 5.15 and 2.8-fold higher than those in the EB population, respectively. Quantitative real-time PCR (qRT-PCR) analysis demonstrated that the mRNA expression levels of CarE and GST genes were positively correlated with the LD50 in the WB, Siziwang Banner (SB) and EB populations. Our findings suggest that differences in susceptibility to pyrethroids in O. asiaticus might be attributed to the elevated activities and mRNA expression levels of CarE and GST genes. (C) 2015 Elsevier Inc. All rights reserved.