A carbon dot-based "off-on" fluorescent probe for highly selective and sensitive detection of phytic acid
A carbon dot-based "off-on" fluorescent probe for highly selective and sensitive detection of phytic acid
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基于碳点的“关断”荧光探针，用于高选择性和灵敏地检测植酸
DOI：
10.1016/j.bios.2015.03.043
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发表时间：
2015-08-15
影响因子：
12.6
通讯作者：
He, Zhimin
中科院分区：
文献类型：
--
作者：
Gao, Zhao;Wang, Libing;He, Zhimin
本文以柠檬酸为碳源，赖氨酸为表面钝化剂，采用一步热解法合成了光致发光碳量子点。结果表明，所制备的荧光粉粒径分布窄，具有良好的蓝色荧光和光稳定性及水分散性。CD的荧光被发现通过高选择性的铁离子（Fe（III））经由光诱导电子转移（PET）过程有效地猝灭。在向CD/Fe（III）络合物分散体中加入植酸（PA）后，CD的荧光显著恢复，这是由于Fe（III）离子从CD/Fe（III）络合物中释放，因为PA与CD相比对Fe（III）离子具有更高的亲和力。此外，我们开发了一种“关-开”荧光分析方法，用于检测植酸使用CD/Fe（III）作为荧光探针。该探针能够选择性检测PA，线性范围为0.68-18.69 μ M，检测限（信噪比为3）为0.36 μ M。该方法对标准品和真实的PA样品的检测具有较高的选择性、重复性、稳定性和回收率。我们认为，这种基于CD的“关-开”荧光探针具有操作简单、成本低、灵敏度高和选择性好等优点，是检测PA的理想传感平台。(C)2015 Elsevier B. V.版权所有。
We herein report a facile, one-step pyrolysis synthesis of photoluminescent carbon dots (CDs) using citric acid as the carbon source and lysine as the surface passivation reagent. The as-prepared CDs show narrow size distribution, excellent blue fluorescence and good photo-stability and water dispersivity. The fluorescence of the CDs was found to be effectively quenched by ferric (Fe(III)) ions with high selectivity via a photo-induced electron transfer (PET) process. Upon addition of phytic acid (PA) to the CDs/Fe(III) complex dispersion, the fluorescence of the CDs was significantly recovered, arising from the release of Fe(III) ions from the CDs/Fe(III) complex because PA has a higher affinity for Fe(III) ions compared to CDs. Furthermore, we developed an "off-on" fluorescence assay method for the detection of phytic acid using CDs/Fe(III) as a fluorescent probe. This probe enables the selective detection of PA with a linear range of 0.68-18.69 mu M and a limit of detection (signal-to-noise ratio is 3) of 0.36 mu M. The assay method demonstrates high selectivity, repeatability, stability and recovery ratio in the detection of the standard and real PA samples. We believe that the facile operation, low-cost, high sensitivity and selectivity render this CD-based "off-on" fluorescent probe an ideal sensing platform for the detection of PA. (C) 2015 Elsevier B.V. All rights reserved.