A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses.
A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses.
复制标题
DOI：
10.1038/srep44778
复制
发表时间：
2017-03-20
影响因子：
4.6
通讯作者：
Meagher RJ
中科院分区：
文献类型：
--
作者：
Priye A;Bird SW;Light YK;Ball CS;Negrete OA;Meagher RJ
目前针对寨卡病毒、登革热病毒和基孔肯雅病毒的多重诊断方法处于可负担性、高性能和适合在资源有限的环境中的护理点使用的交叉点之外。因此，诊断能力不足是目前寨卡疫情管理战略面临的一个关键限制。在这里，我们通过将逆转录环介导等温扩增（RT-LAMP）与我们最近开发的猝灭未掺入扩增信号报告基因（QUASR）技术相结合，展示了寨卡病毒，基孔肯雅病毒和登革热病毒的高度灵敏和特异性检测。我们在一个简单、廉价和便携的“LAMP盒”中进行反应，并辅以消费类智能手机。整个组件可以由5 V USB电源供电，例如USB移动电源或太阳能电池板。我们的智能手机采用了一种利用色度分析荧光信号的新算法，与传统RGB强度传感器或肉眼检测相比，该算法将阳性/阴性信号的区分度提高了5倍。直接从粗制人类样品基质（血液、尿液和唾液）检测ZIKV的能力证明了我们的装置用于广泛临床部署的实用性。总之，这些进步使我们的系统能够托管必要的关键组件，以将基于核酸扩增的检测测定的使用扩展到最需要它们的护理点环境。
Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. Here we demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5 V USB source such as a USB power bank or solar panel. Our smartphone employs a novel algorithm utilizing chromaticity to analyze fluorescence signals, which improves the discrimination of positive/negative signals by 5-fold when compared to detection with traditional RGB intensity sensors or the naked eye. The ability to detect ZIKV directly from crude human sample matrices (blood, urine, and saliva) demonstrates our device’s utility for widespread clinical deployment. Together, these advances enable our system to host the key components necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-care settings where they are needed most.