Cryptic Anuran Biodiversity in Bangladesh Revealed by Mitochondrial 16S rRNA Gene Sequences
Cryptic Anuran Biodiversity in Bangladesh Revealed by Mitochondrial 16S rRNA Gene Sequences
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DOI：
10.2108/zsj.29.162
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发表时间：
2012-03-01
影响因子：
0.9
通讯作者：
Sumida, Masayuki
中科院分区：
文献类型：
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作者：
Hasan, Mahmudul;Islam, Mohammed Mafizul;Sumida, Masayuki
为了调查孟加拉国无尾目物种的多样性，我们比较了 107 个孟加拉国青蛙标本的线粒体 16S rRNA 基因序列（约 1.4 kbp）。遗传分化和系统发育分析的结果结合了相关物种的数据，揭示了至少八种神秘物种的存在。来自两个地区的Hoplobatrachus Tigerinus 差异很大，表明存在一种神秘物种。两个 Fejervarya sp。 （大型和中型）和 Hylarana cf. taipehensis 形成了与相关物种不同的谱系，并且可能是新物种。饰纹藻 cf. ornata 与 M. ornata 在模式产地方面有所不同，并且涉及两个不同的物种。此外，我们还发现了 Hylarana sp。和饰纹藻 sp。与迄今为止在形态学或 16S rRNA 序列上检查的同系物不匹配。初步推测M. fissipes 的存在。因此，本研究中至少发现了 19 个来自孟加拉国的物种。这些发现揭示了孟加拉国丰富的无尾目生物多样性，考虑到该国相当简单的地形特征，这是出乎意料的。
To survey the diversity of anuran species in Bangladesh, we compared mitochondrial 16S rRNA gene sequences (approximately 1.4 kbp) from 107 Bangladesh frog specimens. The results of genetic divergence and phylogenetic analyses incorporating data from related species revealed the occurrence of at least eight cryptic species. Hoplobatrachus tigerinus from two districts diverged considerably, indicating the involvement of a cryptic species. Two Fejervarya sp. (large and medium types) and Hylarana cf. taipehensis formed lineages distinct from related species and are probably new species. Microhyla cf. ornata differed from M. ornata with respect to type locality area and involved two distinct species. In addition, we found that Hylarana sp. and Microhyla sp. did not match congeners examined to date in either morphology or 16S rRNA sequence. The occurrence of M. fissipes was tentatively suggested. Consequently, at least, 19 species were found from Bangladesh in this study. These findings revealed a rich anuran biodiversity in Bangladesh, which is unexpected considering the rather simple topographic features of the country.