MORPHOMETRIC STUDY OF THE GLOMERULAR POPULATION IN THE MOUSE OLFACTORY-BULB - NUMERICAL DENSITY AND SIZE DISTRIBUTION ALONG THE ROSTROCAUDAL AXIS
MORPHOMETRIC STUDY OF THE GLOMERULAR POPULATION IN THE MOUSE OLFACTORY-BULB - NUMERICAL DENSITY AND SIZE DISTRIBUTION ALONG THE ROSTROCAUDAL AXIS
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DOI：
10.1002/cne.902700409
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发表时间：
1988-04-22
影响因子：
2.5
通讯作者：
PLOYE, H
中科院分区：
文献类型：
--
作者：
ROYET, JP;SOUCHIER, C;PLOYE, H
用体视学方法对小鼠嗅球内的肾小球进行了形态计量学研究。在假设肾小球群体是多分散的球体系统的基础上，通过轮廓测量获得的肾小球轮廓分布经过数学展开过程以获得实际的肾小球大小分布。我们使用一种无分布的方法来解释由于截面厚度和截断(两种缺失轮廓机制)而导致的过度投影的综合影响。结果证明比没有体视学分析的轮廓测量直接获得的结果要好。得到了几个新的发现。首先，肾小球的大小沿吻尾轴有显著的变化。嗅球中部的肾小球较大，而其数密度在同一区域减小。此外，剖面密度沿旋转尾轴是均匀的。换句话说，肾小球周围细胞在肾小球层中占据的相对表面是不变的。因此，可以得出结论，大小和数密度的变化是负相关的。因此，由于肾小球在中间区域较大，其单位体积的数量在同一区域逻辑上较小。最后，所有这些数据的计算机化使我们估计了肾小球的数量(1,810。+-。41)在老鼠的嗅球中。为了比较它们的优势和劣势，本研究使用了其他标准的体视学方法来确定这个数字。结合在其他地方获得的个体发育和形态功能数据，讨论了嗅球沿喙尾轴的大小和数密度变化的功能解释。
A morphometric study of the glomerular population in the olfactory bulb of the mouse had been carried out by using stereological methods. On the basis of the assumption that the glomerular population is a polydispersed system of spheres, glomerular profile distributions obtained from profile measurements were subjected to a mathematical unfolding procedure to obtain the actual glomerular size distribution. We used a distribution-free method to account for the combined effects of overprojection due to section thickness and truncation (two missing profile mechanisms). Results proved better than those obtained directly from profile measurements without stereological analysis. Several new findings were obtained. First, significant variations of the glomerulus sizes were found along the rostrocaudal axis. The glomeruli are larger in the middle region of the olfactory bulb, whereas their numerical density decreases in the same region. Moreover, the profile density is homogeneous along the rostrocaudal axis. In other words, the relative surface occupied by the perioglomerular cells in the glomerular layer is invariant. As a consequence, it may be concluded that the varations in size and numerical density are inversely correlated. Thus, since the glomeruli are larger in the middle region, their number per unit volume is logically smaller in this same area. Finally, the computerization of all these data has led us to estimate the number of glomeruli (1,810 .+-. 41) in the olfactory bulb of the mouse. In order to get a comparative idea of their advantages and disadvantages, other standard stereological methods were used in the present study to determine this number. Functional interpretations of the variations of the size and numerical density along the rostrocaudal axis of the olfactory bulb are discussed with respect to ontogenetic and morphofunctional data obtained elsewhere.