Interspecific divergence of circadian properties in duckweed plants
Interspecific divergence of circadian properties in duckweed plants
复制标题
DOI：
10.1111/pce.14297
复制
发表时间：
2022-03-08
影响因子：
7.3
通讯作者：
Oyama, Tokitaka
中科院分区：
文献类型：
--
作者：
Isoda, Minako;Ito, Shogo;Oyama, Tokitaka
生物钟系统在植物中广泛保守；然而，人们对昼夜节律特性的差异知之甚少。我们对近缘浮萍的昼夜节律特性进行了比较分析。利用粒子轰击的方法，将昼夜节律生物发光报告引入浮萍植物。我们测量了浮萍属8种和沃尔菲氏菌属7种在不同温度(20、25和30摄氏度)和光照条件(恒光或恒暗)下的生物发光昼夜节律。Wolffiella种生活在相对温暖的地区，缺少与Lemna种相同的一些组织/器官。浮萍在所有条件下都表现出较强的生物发光昼夜节律，而沃尔菲氏菌则表现出较低的节律稳定性，特别是在较高的温度下。对于浮萍，形成一个支系的两个种(L.valdiana和L.minuta)的昼夜节律稳定性相对较低。对于Wolffiella，形成一个支系的两个种(W.hyalina和W.epanda)表现出极长的周期长度。这些分析表明，物种的昼夜节律特征主要反映了它们的系统发育地位。文中还提出了地理因素和形态因素与昼夜节律的关系。
The circadian clock system is widely conserved in plants; however, divergence in circadian rhythm properties is poorly understood. We conducted a comparative analysis of the circadian properties of closely related duckweed species. Using a particle bombardment method, a circadian bioluminescent reporter was introduced into duckweed plants. We measured bioluminescence circadian rhythms of eight species of the genus Lemna and seven species of the genus Wolffiella at various temperatures (20, 25, and 30 degrees C) and light conditions (constant light or constant dark). Wolffiella species inhabit relatively warm areas and lack some tissues/organs found in Lemna species. Lemna species tended to show robust bioluminescence circadian rhythms under all conditions, while Wolffiella species showed lower rhythm stability, especially at higher temperatures. For Lemna, two species (L. valdiviana and L. minuta) forming a clade showed relatively lower circadian stability. For Wolffiella, two species (W. hyalina and W. repanda) forming a clade showed extremely long period lengths. These analyses reveal that the circadian properties of species primarily reflect their phylogenetic positions. The relationships between geographical and morphological factors and circadian properties are also suggested.