Chemical fingerprint analysis of Phellodendri Amurensis Cortex by ultra performance LC/Q-TOF-MS methods combined with chemometrics
Chemical fingerprint analysis of Phellodendri Amurensis Cortex by ultra performance LC/Q-TOF-MS methods combined with chemometrics
复制标题
超高效LC/Q-TOF-MS方法结合化学计量学分析黄柏皮层化学指纹图谱
DOI：
10.1002/jssc.201000426
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发表时间：
2010-11-01
影响因子：
3.1
通讯作者：
Liu, Changxiao
中科院分区：
文献类型：
--
作者：
Li, Yubo;Zhang, Tiejun;Liu, Changxiao
首次建立了超高效液相色谱-四极杆飞行时间质谱（UPLC/Q-TOF-MS）指纹图谱，用于山茱萸皮（PAC）中主要成分的鉴定。使用沃茨ACQUITY UPLC BEH C18（2.1 x 50 mm，1.7 μ m）通过线性梯度洗脱分离PAC样品，使用水（含0.2%甲酸）和乙腈（含0.2%甲酸）作为移动的相。选取10批PAC样品构建UPLC/Q-TOF-MS指纹图谱。共得到16个共有峰，其中10个峰经文献鉴定，分别为川楝素、木兰花碱、四氢药根碱、野罂粟碱、四氢巴马汀、药根碱、巴马汀、小檗碱、欧乌头酮和柠檬苦素。基于UPLC/Q-TOF-MS图谱中峰的特征，化学计量学方法也被用于评估草药和其他密切相关的草药的变化。指纹图谱分析法可用于PAC的质量控制。
Ultra performance LC with quadrupole TOF MS (UPLC/Q-TOF-MS) fingerprinting is first developed for the identification of the major components of Phellodendri Amurensis Cortex (PAC). The PAC samples are separated using a Waters ACQUITY UPLC BEH C18 (2.1 x 50 mm, 1.7 mu m) by linear gradient elution using water (containing 0.2% formic acid) and acetonitrile (containing 0.2% formic acid) as the mobile phase. Ten batches of PAC are selected to construct the UPLC/Q-TOF-MS fingerprint. Sixteen common peaks in the fingerprint are obtained, ten of which are tentatively identified, with reference to the literature data, as phellodendrine, magnoflorine, tetrahydropjatrorrhizine, menisperine, tetrahydropalmatine, jatrorrhizine, palmatine, berberine, obacunone, and limonin. Chemometric methods are also employed to evaluate the variation of herbal drugs and other closely related herbs based on the characteristics of peaks in the UPLC/Q-TOF-MS profiles. The developed fingerprint assay is a powerful method that may be used to conduct quality control of PAC.