Comparative analysis of 5,211 leaf ESTs of wild rice (Oryza minuta)
Comparative analysis of 5,211 leaf ESTs of wild rice (Oryza minuta)
复制标题
DOI：
10.1007/s00299-004-0764-4
复制
发表时间：
2004-06-01
影响因子：
6.2
通讯作者：
Shin, JS
中科院分区：
文献类型：
--
作者：
Cho, SK;Ok, SH;Shin, JS
所表达的序列标签（est）是野生稻的首个转录组。以栽培4周的米塔（Oryza minuta）叶片为材料，构建了cDNA文库。5211个O. minuta cDNA克隆包含3401个独特序列，包括2787个单子序列和614个组装序列。使用BLAST对GenBank非冗余数据库中的cdna进行数据库比较，发现5,211个cdna中有4,957个（95.1%）与其他生物的基因具有高度的序列同源性。大多数转录本是与代谢、能量、蛋白质生物合成和亚细胞定位相关的基因。在代谢类和能量类est中，玉米est的基因表达量明显高于玉米est。这些数据和基因可用于水稻育种。
The expressed sequence tags (ESTs) presented in this report are the first transcriptomes of wild rice. A cDNA library was constructed from 4-week-old leaf samples of greenhouse-grown Oryza minuta. The 5,211 cDNA clones of O. minuta represent 3,401 unique sequences, consisting of 2,787 singletons and 614 assembled sequences. Database comparisons of the cDNAs in GenBank's non-redundant databases using BLAST revealed that 4,957 of the 5,211 cDNAs (95.1%) showed a high degree of sequence homology to genes from other organisms. Most of the transcripts identified were genes related to metabolism, energy, protein biosynthesis and subcellular localization. The metabolism and energy categories of the O. minuta ESTs showed a considerably higher gene expression level than those of O. sativa ESTs. These data and genes can be utilized in rice breeding.