Optomechanically Actuated Hydrogel Platform for Cell Stimulation with Spatial and Temporal Resolution.
Optomechanically Actuated Hydrogel Platform for Cell Stimulation with Spatial and Temporal Resolution.
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DOI：
10.1021/acsbiomaterials.3c00516
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发表时间：
2023-09-11
影响因子：
5.8
通讯作者：
Salaita, Khalid
中科院分区：
文献类型：
--
作者：
Ramey-Ward, Allison N.;Dong, Yixiao;Yang, Jin;Ogasawara, Hiroaki;Bremer-Sai, Elizabeth C.;Brazhkina, Olga;Franck, Christian;Davis, Michael;Salaita, Khalid
细胞存在于机械动态环境中的体内，但绝大多数体外细胞培养是在静态材料如塑料皿和凝胶上进行的。为了解决这一限制，我们报告了一种方法，广泛使用的水凝胶过渡到机械活性基板内的聚合物基体掺杂光机械致动器（OMA）纳米粒子。OMA由金纳米棒组成，周围环绕着一个温敏聚合物外壳，在近红外（NIR）照射下迅速塌陷。作为概念验证，我们将OMA交联成层粘连蛋白-明胶水凝胶，通过NIR脉冲触发产生高达5 μm的变形。该响应可通过凝胶内的NIR强度和OMA密度来调节，并且可推广到其他水凝胶材料。水凝胶机械刺激增强C2 C12成肌细胞中的肌生成，如ERK信号传导、肌细胞融合和肌节肌球蛋白表达所证明。我们还证明了在慢性炎症模型中由于机械刺激而挽救的分化。这项工作建立了OMA驱动的生物材料作为一个强大的工具，在机械生物学领域的广泛应用，在体外机械操作。
Cells exist in the body in mechanically dynamic environments, yet the vast majority of in vitro cell culture is conducted on static materials such as plastic dishes and gels. To address this limitation, we report an approach to transition widely used hydrogels into mechanically active substrates by doping optomechanical actuator (OMA) nanoparticles within the polymer matrix. OMAs are composed of gold nanorods surrounded by a thermoresponsive polymer shell that rapidly collapses upon near-infrared (NIR) illumination. As a proof of concept, we crosslinked OMAs into laminin-gelatin hydrogels, generating up to 5 μm deformations triggered by NIR pulsing. This response was tunable by NIR intensity and OMA density within the gel and is generalizable to other hydrogel materials. Hydrogel mechanical stimulation enhanced myogenesis in C2C12 myoblasts as evidenced by ERK signaling, myocyte fusion, and sarcomeric myosin expression. We also demonstrate rescued differentiation in a chronic inflammation model as a result of mechanical stimulation. This work establishes OMA-actuated biomaterials as a powerful tool for in vitro mechanical manipulation with broad applications in the field of mechanobiology.
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影响因子：
7.8
作者：
CHARONIS, AS;TSILIBARY, EC;FURTHMAYR, H
通讯作者：
FURTHMAYR, H
影响因子：
7.8
作者：
Belkin, AM;Zhidkova, NI;Balzac, F;Altruda, F;Tomatis, D;Maier, A;Tarone, G;Koteliansky, VE;Burridge, K
通讯作者：
Burridge, K
影响因子：
16.6
作者：
Andreu I;Falcones B;Hurst S;Chahare N;Quiroga X;Le Roux AL;Kechagia Z;Beedle AEM;Elosegui-Artola A;Trepat X;Farré R;Betz T;Almendros I;Roca-Cusachs P
通讯作者：
Roca-Cusachs P
影响因子：
16.6
作者：
Beroz F;Jawerth LM;Münster S;Weitz DA;Broedersz CP;Wingreen NS
通讯作者：
Wingreen NS
影响因子：
4.1
作者：
Egusa, Hiroshi;Kobayashi, Munemasa;Yatani, Hirofumi
通讯作者：
Yatani, Hirofumi