MACROPHAGE SUBPOPULATIONS IN LAMINA PROPRIA OF NORMAL AND INFLAMED COLON AND TERMINAL ILEUM
MACROPHAGE SUBPOPULATIONS IN LAMINA PROPRIA OF NORMAL AND INFLAMED COLON AND TERMINAL ILEUM
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DOI：
10.1136/gut.30.6.826
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发表时间：
1989-06-01
期刊：
影响因子：
24.5
通讯作者：
JEWELL, DP
中科院分区：
文献类型：
--
作者：
MAHIDA, YR;PATEL, S;JEWELL, DP
本研究的目的是描述正常及发炎的回肠和结肠中的人类肠道巨噬细胞的特征。使用一组单克隆抗体进行免疫过氧化物酶染色，并对酸性磷酸酶和非特异性酯酶进行组织化学染色。在浅固有层中，正常结肠巨噬细胞比正常回肠末端的巨噬细胞更大，且酸性磷酸酶和非特异性酯酶的阳性反应更强。在回肠末端浅固有层中有更多的巨噬细胞被单克隆抗体RFD1染色。对炎症性肠病组织的研究表明，存在被单克隆抗体RFD9和3G8染色的巨噬细胞，这些细胞在正常组织中很少出现，并且呈现出与感染性结肠炎不同的模式。对分离的肠道巨噬细胞的研究证实了组织切片中的发现。肠道巨噬细胞亚群可能具有不同的功能作用。炎症期间的表型变化可能是由炎症介质诱导的，也可能代表最近招募的一群细胞。
The aim of this study was to characterise human intestinal macrophages in normal and inflamed ileum and colon. Immunoperoxidase staining with a panel of monoclonal antibodies and histochemical staining for acid phosphatase and non-specific esterase was performed. In the superficial lamina propria, normal colonic macrophages were larger and more strongly positive for acid phosphatase and non-specific esterase than those in normal terminal ileum. There were more macrophages staining with monoclonal antibody RFD1 in the superficial lamina propria of the latter. Studies in inflammatory bowel disease tissue showed the presence of macrophages staining with monoclonal antibodies RFD9 and 3G8 which were rarely present in normal tissue and represented a different pattern from that seen in infectious colitis. Studies on isolated intestinal macrophages confirmed the findings in tissue sections. Subpopulations of intestinal macrophages are likley to have different functional roles. Phenotypic changes during inflammation may be induced by mediators of inflammation or may represent a recently recruited population of cells.