Puerarin attenuates severe burn-induced acute myocardial injury in rats
Puerarin attenuates severe burn-induced acute myocardial injury in rats
复制标题
葛根素减轻大鼠严重烧伤引起的急性心肌损伤
DOI：
10.1016/j.burns.2015.06.001
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发表时间：
2015-12-01
期刊：
影响因子：
2.7
通讯作者：
Wang, Yong-Jie
中科院分区：
文献类型：
--
作者：
Liu, Sheng;Ren, Hong-Bo;Wang, Yong-Jie
背景资料：葛根素（Puerarin）是从葛根（Pueraria lobata）根中提取的主要苷类成分，在我国被广泛用于心血管疾病患者。材料与方法：健康成年Wistar大鼠随机分为3组：（1）假烧伤组，（2）烧伤组，Ⅲ度烧伤面积大于30%，用乳酸林格氏液复苏;烧伤加葛根素组，Ⅲ度烧伤面积大于30%TBSA，用含葛根素的乳酸林格液复苏。分别于伤后1、3、6、12、24 h处死动物。检测血清肌酸激酶同工酶（CK-MB）活性和心肌肌钙蛋白T（cTNT）水平，评价心肌损伤程度。透射电镜观察心肌细胞超微结构的变化。放射免疫法测定血清肿瘤坏死因子（TNF）-α浓度。检测心肌髓过氧化物酶（MPO）活性和丙二醛（MDA）浓度，以分别确定中性粒细胞浸润和氧化应激。结果：30% TBSA Ⅲ度烧伤后，大鼠血清CK MB活性和cTnT水平明显升高，葛根素治疗后，二者均明显降低。烧伤组各时间点血清TNF-α浓度均明显高于假手术组（1.09 ± 0.09ng/ml），伤后12 h达到峰值。烧伤后心肌组织超微结构恶化，MPO活性和MDA含量升高，p38 MAP激酶明显激活。葛根素可改善严重烧伤后心肌细胞超微结构的改变，降低血清TNF-α浓度，抑制心肌组织MPO活性，降低MDA含量，并抑制心肌组织p38 MAP激酶的激活。这些结果表明，葛根素减弱炎症反应，减少中性粒细胞浸润和心脏中的氧化应激，对严重烧伤引起的急性心肌损伤有保护作用。(C)2015 Elsevier Ltd和ISBI。All rights reserved.
Background: Puerarin, the main isoflavone glycoside extracted from the root of Pueraria lobata, is widely prescribed for patients with cardiovascular disorders in China. This study investigates the effect of puerarin on severe burn-induced acute myocardial injury in rats and its underlying mechanisms.Materials and methods: Healthy adult Wistar rats were divided into three groups: (1) sham group, sham burn treatment; (2) burn group, third-degree burns over 30% of the total body surface area (TBSA) with lactated Ringer's solution for resuscitation; and (3) bum plus puerarin group, third-degree burns over 30% of TBSA with lactated Ringer's solution containing puerarin for resuscitation. The burned animals were sacrificed at 1, 3, 6, 12, and 24 h after burn injury. Myocardial injury was evaluated by analyzing serum creatine kinase MB fraction (CK-MB) activity and cardiac troponin T (cTNT) level. Changes in cardiomyocyte ultrastructure were also determined using a transmission electron microscope. Tumor necrosis factor (TNF)-alpha concentration in serum was measured by radioimmunoassay. Cardiac myeloperoxidase (MPO) activity and malondialdehyde (MDA) concentration were measured to determine neutrophil infiltration and oxidative stress in the heart, respectively. The expression of p38 mitogen-activated protein (MAP) kinase in the heart was determined by Western blot analysis.Results: After the 30% TBSA full-thickness bum injury, serum CK-MB activities and cTnT levels increased markedly, both of which were significantly decreased by the puerarin treatment. The level of serum TNF-alpha concentration in burn group at each time-point was obviously higher than those in sham group (1.09 +/- 0.09 ng/ml), and it reached the peak value at 12 h post burn. Burn trauma also resulted in worsen ultrastructural condition, elevated MPO activity and MDA content in heart tissue, and a significant activation of cardiac p38 MAP kinase. Administration of puerarin improved the ultrastructural changes in cardiomyocytes, decreased TNF-alpha concentration in serum as well as suppressed cardiac MPO activity and reduced MDA content, and abolished the activation of p38 MAP kinase in heart tissue after severe bum.Conclusions: These results suggest that puerarin attenuates inflammatory responses, reduces neutrophil infiltration and oxidative stress in the heart, and protects against acute myocardial injury induced by severe burn. (C) 2015 Elsevier Ltd and ISBI. All rights reserved.