Oct-2 DNA binding transcription factor: functional consequences of phosphorylation and glycosylation
Oct-2 DNA binding transcription factor: functional consequences of phosphorylation and glycosylation
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DOI：
10.1093/nar/gkj401
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发表时间：
2006-01-01
影响因子：
14.9
通讯作者：
Nasir-ud-Din
中科院分区：
文献类型：
--
作者：
Ahmad, I;Hoessli, DC;Nasir-ud-Din
磷酸化和O-GlcNAc修饰通常会引起构象变化，并允许蛋白质特异性地与其他蛋白质相互作用。磷酸化和O-GlcNAc修饰在同一保守位点的相互作用可能导致蛋白质发生功能开关。我们描述了在人类Oct-2的保守的Ser/Thr残基上，可以用yinnoyang 1.2方法预测选择性磷酸化和O-GlcNAc修饰（阴阳位点）。我们在此提出，Oct-2与八聚体DNA基序的不同结合行为涉及到n端Ser191、两个POU子结构域连接区域的Ser271和274以及POU子结构域的Thr301和Ser323的选择性磷酸化和O-GlcNAc修饰。这表明同一氨基酸的磷酸化或O-GlcNAc修饰可能导致修饰蛋白的结合能力不同。在c端结构域，Ser371、389和394是可能参与Oct-2结合特性调节的另外的阴阳位点。
Phosphorylation and O-GlcNAc modification often induce conformational changes and allow the protein to specifically interact with other proteins. Interplay of phosphorylation and O-GlcNAc modification at the same conserved site may result in the protein undergoing functional switches. We describe that at conserved Ser/Thr residues of human Oct-2, alternative phosphorylation and O-GlcNAc modification (Yin Yang sites) can be predicted by the YinOYang 1.2 method. We propose here that alternative phosphorylation and O-GlcNAc modification at Ser191 in the N-terminal region, Ser271 and 274 in the linker region of two POU sub-domains and Thr301 and Ser323 in the POUh subdomain are involved in the differential binding behavior of Oct-2 to the octamer DNA motif. This implies that phosphorylation or O-GlcNAc modification of the same amino acid may result in a different binding capacity of the modified protein. In the C-terminal domain, Ser371, 389 and 394 are additional Yin Yang sites that could be involved in the modulation of Oct-2 binding properties.