Differential expression of KDR/VEGFR-2 and CD34 during mesoderm development of the early human embryo
Differential expression of KDR/VEGFR-2 and CD34 during mesoderm development of the early human embryo
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DOI：
10.1016/s0925-4773(99)00030-1
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发表时间：
1999-05-01
影响因子：
2.6
通讯作者：
Labastie, MC
中科院分区：
文献类型：
--
作者：
Cortés, F;Debacker, C;Labastie, MC
最近对脊椎动物胚胎的研究结果为成血管细胞的存在提供了令人信服的证据，即内皮细胞和造血细胞的共同前体，其特征在于表达VEGFR 2/Flk 1受体。我们在这里描述了一个人口的KDR+ CD 34（-）中胚层细胞出现在早期犹太人胚胎，由妊娠第4周开始。在发育中的血管中，表达KDR的CD 34（-）细胞逐渐共表达增加水平的CD 34抗原。值得注意的是，随着发育的进行，KDR+ CD 34（-）特遣队持续存在于腹主动脉旁的内脏胸膜，直到出现与造血相关的造血细胞簇之前。这些观察结果表明，KDR+ CD 34（-）中胚层细胞可能代表了假定的人造血和内皮细胞系的血管母细胞前体。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
Recent findings on vertebrate embryos have provided compelling evidence for the existence of hemangioblasts, i.e. common precursors for endothelial and hematopoietic cells, characterized by expression of the VEGFR2/Flk1 receptor. We describe here a population of KDR+ CD34(-) mesoderm cells that emerges in early-semitic human embryos, by the beginning of the 4th week of gestation. In the developing blood vessels, KDR-expressing CD34(-) cells gradually coexpress increasing levels of CD34 antigen. Remarkably, as development proceeds, a KDR+ CD34(-) contingent persists in the paraaortic splanchnopleura until just prior to the emergence of aorta-associated hematopoietic cell clusters. These observations suggest that KDR+ CD34(-) mesodermal cells might represent the putative hemangioblastic precursor of human hematopoietic and endothelial lineages. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.