An electron‐ and light‐microscope study of sinus structure in perfused rabbit and dog spleens
An electron‐ and light‐microscope study of sinus structure in perfused rabbit and dog spleens
复制标题
兔和狗灌注脾脏窦结构的电子和光学显微镜研究
DOI：
10.1002/aja.1001200307
复制
发表时间：
1967
影响因子：
--
通讯作者：
C. E. Thomas
中科院分区：
文献类型：
--
作者：
C. E. Thomas
通过低压灌注去除血细胞，可以在兔和狗的脾脏中产生清晰的窦壁视图。窦壁包含三个部分：内层窦细胞、中间层基底膜和外层索限制细胞。窦细胞和索限制细胞在细胞质细节上显然彼此相似，似乎都是网状细胞，仅具有有限的吞噬金颗粒的能力。窦壁上的孔使血细胞能够穿过壁。灌注脾脏中的许多孔不含细胞，但仍保持开放。窦细胞通常是连续的并且通常不与孔交替。然而，窦细胞交叉的现象很轻微，窦细胞之间紧密连接和桥粒的证据尚无定论。
The removal of blood cells by low-pressure perfusion produced well-delineated views of the sinus wall in rabbit and dog spleens. The sinus wall contained three elements: an inner layer of sinus cells, an intermediate layer of basement membrane, and an outer layer of cord-limiting cells. Sinus and cord-limiting cells, apparently similar to each other in cytoplasmic detail, appeared to be reticular cells that possessed only a limited capacity for phagocytosis of gold particles. Apertures in the sinus wall enabled blood cells to pass through the wall. Many apertures in perfused spleen contained no cells and yet remained patent. Sinus cells often were contiguous and commonly did not alternate with apertures. However, sinus-cell interdigitation was slight, and evidence for tight junctions and desmosomes between sinus cells was inconclusive.