Integrated analysis of serum and liver metabonome in liver transplanted rats by gas chromatography coupled with mass spectrometry
Integrated analysis of serum and liver metabonome in liver transplanted rats by gas chromatography coupled with mass spectrometry
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气相色谱-质谱联用分析肝移植大鼠血清和肝脏代谢组
DOI：
10.1016/j.aca.2008.11.034
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发表时间：
2009-02-02
影响因子：
6.2
通讯作者：
Cheng, Yiyu
中科院分区：
文献类型：
--
作者：
Wang, Yi;Tao, Yi;Cheng, Yiyu
本文介绍了一种代谢组学方法，用于研究肝移植大鼠血清和肝组织中的异常代谢过程。对雄性刘易斯大鼠进行同基因移植。收集移植后第1、3和7天的血清和移植肝脏，使用气相色谱-质谱联用（GC-MS）分析内源性代谢物。该方法经验证具有可接受的线性、精密度和重复性。在血清和肝脏中分别鉴定出34种和29种代谢物。相关性分析结果表明，功能相似的代谢产物在肝脏和血清中的变化规律相似。主成分分析（PCA）处理的数据显示时间依赖性的生化变化。因此，本研究可能为原位肝移植的病理生理机制提供特定的推定途径。(C)2008 Elsevier B. V.保留所有权利。
In this paper, we present a metabonomic method for the investigation of abnormal metabolic process in both serum and liver tissue of liver transplanted rats. Syngeneic transplantation was performed on male Lewis rats. The serum and grafted liver on day 1,3,and 7 post-transplant were collected to analyze endogenous metabolites using gas chromatography coupled with mass spectrometry (GC-MS). The method was validated with acceptable linearity, precision, and repeatability. Thirty-four metabolites in serum and 29 metabolites in liver were identified. Results of correlation analysis illustrated metabolites with similar function exhibited similar variations in liver and serum. The data processed by principle component analysis (PCA) showed time-dependent biochemical variations. As a consequence, the present study may offer specific putative pathways in the pathophysiological mechanism of orthotopic liver transplantation. (C) 2008 Elsevier B.V. All rights reserved.