Analyzing Polyaniline-poly(2-acrylamido-2-methylpropane sulfonic acid) Biocompatibility with 3T3 Fibroblasts
Analyzing Polyaniline-poly(2-acrylamido-2-methylpropane sulfonic acid) Biocompatibility with 3T3 Fibroblasts
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DOI：
10.1163/156856209x434647
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发表时间：
2010-01-01
影响因子：
3.6
通讯作者：
Peppas, Nicholas A.
中科院分区：
文献类型：
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作者：
Bayer, Carolyn L.;Trenchard, Isis J.;Peppas, Nicholas A.
导电聚合物可作为独特的功能元件应用于组织工程、细胞刺激和药物输送等领域的智能生物材料。这些领域的研究可能受到已建立的导电聚合物合成方法的限制，这些方法不能提供大规模加工这些材料的简单途径。通常，导电聚合物在生物相容性溶剂中高度不溶，并且可能是机械脆性的，使得它们与生物材料的整合具有挑战性。作为替代方案，提出了使用水溶性导电聚合物与生物材料集成，聚（2-丙烯酰胺基-2-甲基丙磺酸）（PANI-PAAMPSA）合成的聚苯胺模板。为了解决关于这种导电聚合物的生物相容性的关键基本问题，对在PANI-PAAMPSA膜上培养的NIH 3 T3成纤维细胞进行了细胞毒性和增殖测定。确定细胞培养物保持与对照表面上培养的细胞相似的生长习性。此外，证明了PANI-PAAMPSA膜在暴露于细胞培养物之后的导电性，表明材料在细胞生长之后保留功能性。这些结果表明，这种类型的模板合成聚苯胺可以成功地实现作为一个功能，导电生物材料。(C)Koninklijke Brill NV，莱顿，2010年
Conductive polymers can be utilized as unique functional elements in future intelligent biomaterials required for tissue engineering, cell stimulation and drug delivery. Research in these areas could be limited by established methods of conductive polymer synthesis which do not provide an easy route towards large scale processing of these materials. Typically, conductive polymers are highly insoluble in biocompatible solvents, and can be mechanically brittle, making their integration with biomaterials challenging. As an alternative, the use of a water-soluble conductive polymer for integration with biomaterials, a polyaniline template synthesized with poly(2-acrylamido-2-methylpropanesulfonic acid) (PANI-PAAMPSA), is proposed. To address key fundamental questions about the biocompatibility of this conductive polymer, cell cytotoxicity and proliferation assays have been performed on NIH 3T3 fibroblasts cultured on films of PANI-PAAMPSA. It was determined that the cell cultures maintained growth habits similar to those cultured on the control surfaces. Additionally, conductivity of the PANI-PAAMPSA films subsequent to exposure to the cell culture was demonstrated, indicating the materials retain functionality after cell growth. These results indicate that this type of template synthesized PANI could be successfully implemented as a functional, conductive biomaterial. (C) Koninklijke Brill NV, Leiden, 2010