Functional analysis of a pathogenesis-related thaumatin-like protein gene TaLr35PR5 from wheat induced by leaf rust fungus.
Functional analysis of a pathogenesis-related thaumatin-like protein gene TaLr35PR5 from wheat induced by leaf rust fungus.
复制标题
叶锈菌诱导小麦病程相关索马甜蛋白基因TaLr35PR5的功能分析
DOI：
10.1186/s12870-018-1297-2
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发表时间：
2018-05-04
影响因子：
5.3
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Zhang J;Wang F;Liang F;Zhang Y;Ma L;Wang H;Liu D
植物已经进化出多方面的防御机制来抵抗病原体的感染。病原学相关蛋白（PR）的产生与植物抗病有关，特别是系统获得性抗性（SAR）。我们早期的研究报道了从小麦近等基因系TcLr35中分离到一个全长TaLr35PR5基因，该基因编码一个与甜蛋白thaumatin具有氨基酸和结构相似性的蛋白质。本研究旨在了解TaLr35PR5基因在lr35介导的小麦锈菌成虫抗性中的作用。我们利用酵母信号序列陷阱系统确定了TaLr35PR5蛋白含有一个功能性分泌肽。通过洋葱表皮细胞的异源表达实验，我们发现TaLr35PR5蛋白可以分泌到洋葱细胞的外质体中。在bsmv诱导的基因沉默小麦植株中，TaLr35PR5的表达显著降低，病理检测显示lr35介导的抗性表型明显改变，表明lr35介导的抗性受损。这些结果有力地表明，TaLr35PR5参与了lr35介导的小麦对叶锈病的防御。
Plants have evolved multifaceted defence mechanisms to resist pathogen infection. Production of the pathogenesis-related (PR) proteins in response to pathogen attack has been implicated in plant disease resistance specialized in systemic-acquired resistance (SAR). Our earlier studies have reported that a full length TaLr35PR5 gene, encoding a protein exhibiting amino acid and structural similarity to a sweet protein thaumatin, was isolated from wheat near-isogenic line TcLr35. The present study aims to understand the function of TaLr35PR5 gene in Lr35-mediated adult resistance to Puccinia triticina. We determined that the TaLr35PR5 protein contained a functional secretion peptide by utilizing the yeast signal sequence trap system. Using a heterologous expression assay on onion epidermal cells we found that TaLr35PR5 protein was secreted into the apoplast of onion cell. Expression of TaLr35PR5 was significantly reduced in BSMV-induced gene silenced wheat plants, and pathology test on these silenced plants revealed that Lr35-mediated resistance phenotype was obviously altered, indicating that Lr35-mediated resistance was compromised. All these findings strongly suggest that TaLr35PR5 is involved in Lr35-mediated adult wheat defense in response to leaf rust attack.