Genome-Wide Identification of the B-BOX Genes that Respond to Multiple Ripening Related Signals in Sweet Cherry Fruit.
Genome-Wide Identification of the B-BOX Genes that Respond to Multiple Ripening Related Signals in Sweet Cherry Fruit.
复制标题
甜樱桃果实中响应多种成熟相关信号的B-BOX基因的全基因组鉴定
DOI：
10.3390/ijms22041622
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发表时间：
2021-02-05
影响因子：
5.6
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Wang Y;Zhai Z;Sun Y;Feng C;Peng X;Zhang X;Xiao Y;Zhou X;Wang W;Jiao J;Li T
B-box蛋白是一类锌指转录因子，在植物的生长发育和非生物胁迫反应中发挥重要作用。在本研究中，我们在甜樱桃基因组数据库中鉴定了15个PavBBX基因。我们系统地分析了这些基因在果实发育过程中以及对光和各种激素的响应过程中的基因结构、聚类特征和表达模式。PavBBX基因分为5个亚组。PavBBX基因的启动子区域包含与植物发育、激素和逆境相关的顺式作用元件。QRT-PCR检测到5个PavBBX基因在果实发育过程中表达上调，8个基因表达下调。此外，PavBBX6、PavBBX9和PavBBX11在光诱导下表达上调。我们还发现，ABA、BR和GA3含量在光诱导下显著增加。此外，几个PavBBX基因的表达与花青素生物合成基因、光响应基因和多激素信号通路基因的表达高度相关。部分PavBBX基因受到ABA、GA和BR处理的强烈诱导。值得注意的是，PavBBX6和PavBBX9对所有这三种激素都有反应。综上所述，BBX蛋白可能通过整合光、ABA、GA和BR信号通路，在调节甜樱桃果实花青素的生物合成中发挥重要作用。
B-BOX proteins are zinc finger transcription factors that play important roles in plant growth, development, and abiotic stress responses. In this study, we identified 15 PavBBX genes in the genome database of sweet cherry. We systematically analyzed the gene structures, clustering characteristics, and expression patterns of these genes during fruit development and in response to light and various hormones. The PavBBX genes were divided into five subgroups. The promoter regions of the PavBBX genes contain cis-acting elements related to plant development, hormones, and stress. qRT-PCR revealed five upregulated and eight downregulated PavBBX genes during fruit development. In addition, PavBBX6, PavBBX9, and PavBBX11 were upregulated in response to light induction. We also found that ABA, BR, and GA3 contents significantly increased in response to light induction. Furthermore, the expression of several PavBBX genes was highly correlated with the expression of anthocyanin biosynthesis genes, light-responsive genes, and genes that function in multiple hormone signaling pathways. Some PavBBX genes were strongly induced by ABA, GA, and BR treatment. Notably, PavBBX6 and PavBBX9 responded to all three hormones. Taken together, BBX proteins likely play major roles in regulating anthocyanin biosynthesis in sweet cherry fruit by integrating light, ABA, GA, and BR signaling pathways.