NEUROTROPHINS REGULATE DENDRITIC GROWTH IN DEVELOPING VISUAL-CORTEX
NEUROTROPHINS REGULATE DENDRITIC GROWTH IN DEVELOPING VISUAL-CORTEX
复制标题
DOI：
10.1016/0896-6273(95)90171-x
复制
发表时间：
1995-10-01
期刊：
影响因子：
16.2
通讯作者：
KATZ, LC
中科院分区：
文献类型：
--
作者：
MCALLISTER, AK;LO, DC;KATZ, LC
虽然树突状细胞的生长和分化对新皮层的正常发育和功能至关重要，但调控这些过程的分子信号在很大程度上是未知的。通过用NGF、BDNF、NT-3或NT-4处理发育中的视皮层切片，并随后使用颗粒介导的基因转移使锥体神经元的树突可视化，来测试神经营养因子的潜在作用。特定的神经营养因子增加了确定的细胞群体树突的长度和复杂性。基底树突的神经元在每个皮质层中的反应最强烈的一个单一的神经营养因子：神经元在第4层的BDNF和神经元在第5层和第6层的NT-4。相反，顶端树突对一系列神经营养因子作出反应。在顶端和基底树突上，TrkB受体配体BDNF和NT-4的作用是不同的。神经营养作用的光谱和这些行动的层状特异性暗示内源性神经营养因子作为调节信号在哺乳动物新皮层的特定树突模式的发展。
Although dendritic growth and differentiation are critical for the proper development and function of neocortex, the molecular signals that regulate these processes are largely unknown. The potential role of neurotrophins was tested by treating slices of developing visual cortex with NGF, BDNF, NT-3, or NT-4 and by subsequently visualizing the dendrites of pyramidal neurons using particle-mediated gene transfer. Specific neurotrophins increased the length and complexity of dendrites of defined cell populations. Basal dendrites of neurons in each cortical layer responded most strongly to a single neurotrophin: neurons in layer 4 to BDNF and neurons in layers 5 and 6 to NT-4. In contrast, apical dendrites responded to a range of neurotrophins. On both apical and basal dendrites, the effects of the TrkB receptor ligands, BDNF and NT-4, were distinct. The spectrum of neurotrophic actions and the laminar specificity of these actions implicate endogenous neurotrophins as regulatory signals in the development of specific dendritic patterns in mammalian neocortex.