AN ENZYMATIC METHOD FOR PREPARATION OF DISSOCIATED MURINE PEYERS PATCH CELLS ENRICHED FOR MACROPHAGES
AN ENZYMATIC METHOD FOR PREPARATION OF DISSOCIATED MURINE PEYERS PATCH CELLS ENRICHED FOR MACROPHAGES
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DOI：
10.1016/0022-1759(82)90207-1
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发表时间：
1982-01-01
影响因子：
2.2
通讯作者：
MCGHEE, JR
中科院分区：
文献类型：
--
作者：
FRANGAKIS, MV;KOOPMAN, WJ;MCGHEE, JR
小鼠Peyer' s patches （PP）的酶（解离酶）解离产生丰富的T和B淋巴细胞和含有5-9%巨噬细胞（M.vphi.）的细胞群，由形态学和酯酶染色确定。这种方法的细胞产量是传统机械方法的3-4倍，细胞存活率始终在95%以上。IgA浆细胞的缺失（< 1%）表明细胞制剂未受到肠粘膜细胞的污染。酶解的PP细胞在T细胞和B细胞分裂原的作用下增殖。观察到顺序获得的PP细胞组分有丝分裂原反应模式的差异，表明PP细胞具有相当大的功能异质性。PP细胞对B细胞多克隆激活剂脂多糖的IgM和IgA合成模式与脾细胞有很大不同。制备功能完整的T细胞和B细胞并富集M.vphi的PP细胞的方法的发展。应该允许分析PP功能专门化的细胞基础。
Enzyme (Dispase) dissociation of murine Peyer''s patches (PP) yields a cell population rich in T and B lymphocytes and containing 5-9% macrophages (M.vphi.) as defined by morphology and esterase staining. Cell yields by this method are 3-4 times greater than with conventional mechanical methods and viability of cells always is > 95%. The virtual absence of IgA plasma cells (< 1%) indicates lack of contamination of the cell preparations with intestinal mucosal cells. Enzymatically dissociated PP cells proliferate in response to both T and B cell mitogens. Differences in patterns of mitogen responsiveness of sequentially obtained PP cell fractions are observed, suggesting considerable functional heterogeneity of PP cells. Patterns of IgM and IgA synthesis by PP cells in response to the B cell polyclonal activator lipopolysaccharide differ considerably from spleen cells. The development of methodology for preparation of PP cells containing functionally intact T and B cells and enriched for M.vphi. should permit analysis of the cellular basis for functional specialization of PP.