Brv1 Is Required for Drosophila Larvae to Sense Gentle Touch.
Brv1 Is Required for Drosophila Larvae to Sense Gentle Touch.
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DOI：
10.1016/j.celrep.2018.03.041
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发表时间：
2018-04
期刊：
影响因子：
8.8
通讯作者：
Mingfeng Zhang;Xia Li;Honglan Zheng;Xiaoxu Wen;Sihan Chen;Jia Ye;Siyang Tang;Fuqiang Yao;Yuezhou Li;Zhiqiang Yan
中科院分区：
文献类型：
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作者：
Mingfeng Zhang;Xia Li;Honglan Zheng;Xiaoxu Wen;Sihan Chen;Jia Ye;Siyang Tang;Fuqiang Yao;Yuezhou Li;Zhiqiang Yan
我们如何感知触觉是许多生理过程的基础。然而，触觉的潜在机制和分子特性在很大程度上是未知的。在这里，我们报告了有缺陷的轻触行为反应在果蝇幼虫的功能丧失。RNAi和Ca2+成像证实了Brv1参与感觉触摸，并证明Brv1介导III类树突树突神经元的机械转导。电生理记录进一步显示，Brv1蛋白在HEK293T细胞中的表达产生了拉伸激活的阳离子通道。重组为脂质体的纯化Brv1蛋白被发现能感知拉伸刺激。此外，共表达研究表明，Brv1放大了机械敏感离子通道NOMPC（无机械受体电位C）对触摸刺激的反应。总之，这些发现证明了一种分子实体可以介导果蝇的轻触反应，为触摸感觉的分子机制提供了新的见解。
How we sense touch is fundamental for many physiological processes. However, the underlying mechanism and molecular identity for touch sensation are largely unknown. Here, we report on defective gentle-touch behavioral responses inbrv1loss-of-functionDrosophilalarvae. RNAi and Ca2+imaging confirmed the involvement of Brv1 in sensing touch and demonstrated that Brv1 mediates the mechanotransduction of class III dendritic arborization neurons. Electrophysiological recordings further revealed that the expression of Brv1 protein in HEK293T cells gives rise to stretch-activated cation channels. Purified Brv1 protein reconstituted into liposomes were found to sense stretch stimuli. In addition, co-expression studies suggested that Brv1 amplifies the response of mechanosensitive ion channel NOMPC (no mechanoreceptor potential C) to touch stimuli. Altogether, these findings demonstrate a molecular entity that mediates the gentle-touch response inDrosophilalarvae, providing insights into the molecular mechanisms of touch sensation.