Elevated 22Na uptake in aortae of Dahl salt-sensitive rats with high salt diet.
Elevated 22Na uptake in aortae of Dahl salt-sensitive rats with high salt diet.
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高盐饮食对 Dahl 盐敏感大鼠主动脉 22Na 的摄取升高。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
C. Sampson
中科院分区：
文献类型：
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作者：
S. Vasdev;V. Prabhakaran;C. Sampson
我们研究了高盐摄入量对 Dahl 盐敏感 (DS) 大鼠血压和血管 22Na 摄取的影响。 6 周龄时，一组 6 只 DS 大鼠接受低盐饮食（0.4%），第二组 6 只 DS 大鼠接受高盐饮食（8.0%），持续 4 周。每周记录一次血压。 10周龄时，处死动物并测量主动脉22Na摄取量。测量 22Na 摄取的总成分和阿米洛利敏感成分（Na(+)-H+反向转运），从中计算阿米洛利不敏感成分。在这些血管 22Na 摄取实验中，用哇巴因抑制 Na+、K(+) 泵，以防止 Na+ 外流。与低盐饮食的 DS 大鼠相比，高盐饮食的 DS 大鼠血压显着升高（P 小于 0.01）。同样，与低盐饮食的 DS 大鼠相比，高盐饮食的 DS 大鼠表现出显着（P 小于 0.05）的阿米洛利敏感和阿米洛利不敏感血管 22Na 总摄取量。血管 22Na 摄取量和血压的平行增加表明 Na+ 流入在 DS 大鼠盐诱导高血压机制中可能发挥关键作用。
We examined the effects of high salt intake on blood pressure and vascular 22Na uptake in Dahl salt-sensitive (DS) rats. At 6 weeks of age, one group of 6 DS rats was placed on a low (0.4%) salt diet and the second group of 6 DS rats was placed on a high (8.0%) salt diet for a period of 4 weeks. Blood pressure recordings were made weekly. At 10 weeks of age, the animals were sacrificed and aortic 22Na uptake was measured. Total and amiloride sensitive (Na(+)-H+ antiport) components of 22Na uptake were measured from which was calculated the amiloride insensitive component. Na+, K(+)-pumps were inhibited for these vascular 22Na uptake experiments with ouabain to prevent Na+ efflux. DS rats on the high salt diet demonstrated significantly (P less than 0.01) higher blood pressure when compared to DS rats on a low salt diet. Similarly, DS rats on a high salt diet demonstrated significantly (P less than 0.05) higher total, amiloride sensitive and amiloride insensitive vascular 22Na uptake as compared to DS rats on low salt diet. The parallel increase in vascular 22Na uptake and blood pressure suggests a possible, key role of Na+ influx in the mechanism of salt induced hypertension of DS rats.