Selective cell culture of primary breast carcinoma.
Selective cell culture of primary breast carcinoma.
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发表时间：
1995-06
期刊：
影响因子：
11.2
通讯作者：
S. Dairkee;Guoren Deng;MR Stampfer;Frederic M. Waldman;Helene S. Smith
中科院分区：
文献类型：
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作者：
S. Dairkee;Guoren Deng;MR Stampfer;Frederic M. Waldman;Helene S. Smith
我们使用模拟乳腺肿瘤微环境的培养条件来体外分离和增殖原发性乳腺肿瘤细胞。在这种单层设置中，从原发性乳腺肿瘤分离的细胞混合物受到自创的氧气和营养物以及代谢废物和细胞外 pH 值梯度的影响。在这些新条件下分离的肿瘤群体表现出乳腺癌的表型特征，包括细胞角蛋白 19 的同质表达，以及阳离子染料罗丹明 123 的线粒体保留增加。来自乳房缩小术的非恶性培养物无法在这些条件下存活。达到第 10 代的一个肿瘤群体的 15 号和 17 号染色体为非整倍体，并在外显子 8 中显示出 p53 突变。这些研究强烈表明，本文描述的培养条件可以抑制正常乳腺细胞的生长，从而允许在体外选择性分离一些生长缓慢的原发性肿瘤细胞群体。
We have used culture conditions which simulate the microenvironment of breast tumors for the isolation and propagation of primary breast tumor cells in vitro. In this monolayer setup, the mixture of cells dissociated from primary breast tumors is subjected to self-created gradients of oxygen and nutrients as well as metabolic waste and extracellular pH. The tumor populations isolated under these novel conditions have displayed phenotypic properties characteristic of breast carcinomas, including homogeneous expression of cytokeratin 19, and increased mitochondrial retention of the cationic dye rhodamine 123. Nonmalignant cultures from reduction mammoplasty were unable to survive these conditions. One tumor population which reached passage 10 was aneuploid for chromosomes 15 and 17, and displayed a p53 mutation in exon 8. These studies strongly suggest that the culture conditions described here can suppress the growth of normal breast cells, thereby allowing selective isolation of some populations of slow-growing primary tumor cells in vitro.