The use of the dilute Russell viper venom time for the diagnosis of lupus anticoagulants.
The use of the dilute Russell viper venom time for the diagnosis of lupus anticoagulants.
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DOI：
10.1182/blood.v68.4.869.869
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发表时间：
1986-10
期刊：
影响因子：
20.3
通讯作者：
P. Thiagarajan;V. Pengo;S. Shapiro
中科院分区：
文献类型：
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作者：
P. Thiagarajan;V. Pengo;S. Shapiro
我们在这里描述了一个测试狼疮抗凝剂的基础上修改罗素蝰蛇毒液时间（RVVT），使用有限的磷脂和毒液量。我们研究了29例长期稀释RVVT患者。29人中有5人的活化部分凝血活酶时间正常，14人中有3人的组织凝血活酶抑制试验正常。在19例受试患者中，17例的稀释RVVT是完全正常的，当离子载体处理的血小板取代磷脂，其余两名患者，都非常长的磷脂依赖性稀释RVVT的，几乎完全正常化。在存在因子VIII、IX或XI抗体的情况下，稀释的RVVT不会延长。因此，稀释RVVT似乎是一种简单，可重复性，灵敏度和相对特异性的狼疮抗凝剂的检测方法。
We describe here a test for lupus anticoagulants based on a modified Russell viper venom time (RVVT), using limiting amounts of phospholipid and venom. We have studied 29 patients with a prolonged dilute RVVT. Five of the 29 had a normal activated partial thromboplastin time and three of 14 tested by the tissue thromboplastin inhibition test were normal. In 17 of 19 patients tested, the dilute RVVT was completely normal when ionophore-treated platelets were substituted for phospholipid; the remaining two patients, both with very long phospholipid-dependent dilute RVVT's, were nearly completely normalized. The dilute RVVT is not prolonged in the presence of antibodies to factors VIII, IX, or XI. Thus, the dilute RVVT appears to be a simple, reproducible, sensitive, and relatively specific method for the detection of lupus anticoagulants.