Large Volume Electron Microscopy and Neural Microcircuit Analysis.
Large Volume Electron Microscopy and Neural Microcircuit Analysis.
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DOI:
10.3389/fncir.2018.00098
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发表时间:
2018
影响因子:
3.5
通讯作者:
Kawaguchi Y
中科院分区:
文献类型:
--
作者:
Kubota Y;Sohn J;Kawaguchi Y
One recent technical innovation in neuroscience is microcircuit analysis using three-dimensional reconstructions of neural elements with a large volume Electron microscopy (EM) data set. Large-scale data sets are acquired with newly-developed electron microscope systems such as automated tape-collecting ultramicrotomy (ATUM) with scanning EM (SEM), serial block-face EM (SBEM) and focused ion beam-SEM (FIB-SEM). Currently, projects are also underway to develop computer applications for the registration and segmentation of the serially-captured electron micrographs that are suitable for analyzing large volume EM data sets thoroughly and efficiently. The analysis of large volume data sets can bring innovative research results. These recently available techniques promote our understanding of the functional architecture of the brain.
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