Developmental and tissue-specific regulation of beta-tubulin gene expression in the embryo of the sea urchin Strongylocentrotus purpuratus.
Developmental and tissue-specific regulation of beta-tubulin gene expression in the embryo of the sea urchin Strongylocentrotus purpuratus.
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海胆 Strongylocentrotus purpuratus 胚胎中 β-微管蛋白基因表达的发育和组织特异性调控。
DOI：
10.1101/gad.1.2.147
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发表时间：
1987
影响因子：
10.5
通讯作者：
Nemer,M
中科院分区：
文献类型：
--
作者：
Harlow,P;Nemer,M
海胆（Strongylocentrotus purpuratus）胚胎中的四种β-微管蛋白mRNA由9-12种β-微管蛋白基因中的至少3种转录。由独特的β 1基因转录的1.8 kb的β 1微管蛋白mRNA在pluteus外胚层中以高特异性表达。另一个1.8 kb的mRNA，β 2和2.5 kb的β 3 mRNA是中度外胚层特异性的。相比之下，3.0 kb的β 4 mRNA对内中胚层组织组分具有高度特异性。发育和组织特异性表达的某些相似之处表明，这些β-微管蛋白基因可能在其调控模式中与另一种细胞骨架蛋白肌动蛋白基因中的对应物相关。绝对量的测量揭示了每个β-微管蛋白mRNA的不同发育概况。在早期囊胚中的β-微管蛋白mRNA的总量的增加与每个细胞核的转录率的增加相关;而在间充质囊胚阶段后期，β-微管蛋白mRNA水平急剧下降，因为每个胚胎的β-微管蛋白基因转录率保持不变。因此，在发育过程中通过囊胚阶段，有一个开关，主要是转录后调控β-微管蛋白mRNA的表达，可能是通过降低mRNA的稳定性。
Four beta-tubulin mRNAs in the embryo of the sea urchin Strongylocentrotus purpuratus are transcribed from at least 3 of the 9-12 beta-tubulin genes. A beta 1 tubulin mRNA of 1.8 kb, transcribed from a unique beta 1 gene, is expressed with high specificity in the pluteus ectoderm. Another 1.8-kb mRNA, beta 2, and a 2.5-kb beta 3 mRNA are moderately ectoderm specific. In contrast, a 3.0-kb beta 4 mRNA is highly specific for the endomesoderm tissue fraction. Certain similarities in developmental and tissue-specific expression suggest that these beta-tubulin genes may be related in their mode of regulation to counterparts among the genes for actin, another cytoskeletal protein. Measurements of absolute amounts revealed a distinct developmental profile for each beta-tubulin mRNA. An increase in the total amount of beta-tubulin mRNA in the early blastula was correlated with an increase in transcription rate per nucleus; whereas, later in the mesenchyme blastula stage, the beta-tubulin mRNA level decreased sharply as the rate of beta-tubulin gene transcription on a per embryo basis remained constant. Thus, during development through the blastula stages, there was a switch to a predominantly posttranscriptional regulation of beta-tubulin mRNA expression, probably through a decrease in mRNA stability.