First case of molecularly identified and genetically characterized human T-cell leukemia virus type 2 infection in a pregnant woman in non-endemic Japan
First case of molecularly identified and genetically characterized human T-cell leukemia virus type 2 infection in a pregnant woman in non-endemic Japan
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DOI：
10.1016/j.jviromet.2020.114005
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发表时间：
2021-01-01
影响因子：
3.1
通讯作者：
Hamaguchi, Isao
中科院分区：
文献类型：
--
作者：
Kuramitsu, Madoka;Okuma, Kazu;Hamaguchi, Isao
人类t细胞白血病病毒2型（HTLV-2）与相关的HTLV 1型不同，在日本不是地方性的。此前，虽然在日本一名男性献血者中通过western n - blotting检测出HTLV-2血清阳性，但没有通过核酸检测检测到HTLV-2原病毒的报道。在本报告中，一名日本孕妇在日本通过产前筛查进行初步检测后，通过特异性抗体的直线免疫分析法被临床诊断为htlv -2感染。在其外周血单个核细胞基因组DNA中，采用定量聚合酶链反应核酸检测HTLV-2前病毒基因组（三种方法）。成功地确定了该菌株的全基因组序列。有趣的是，通过重组区和系统发育树分析，鉴定出的病毒被认为是a和c亚型的祖先。总之，据我们所知，目前的感染是日本非地方性的产前筛查发现的第一例分子鉴定和遗传特征的HTLV-2感染。
Human T-cel l leukemia virus type 2 (HTLV-2) is non-endemic in Japan unlike the related HTLV type 1. Previously, although HTLV-2-seropositivity was identified via wester n blottin g in one male blood donor in Japan, there have been no reports of HTLV-2 provirus detection by nucleic acid testing. In this report, one Japanese pregnant woman was clinically diagnosed as being HTLV-2-infected with a line immunoassay for specific antibodies after primary testing through prenatal screening in Japan. In genomic DNA of her peripheral blood mononuclear cells, HTLV-2 proviral genome was detected by nucleic acid testing (three methods) with quantitative polymerase chain reaction. The f ull-genome sequence of this strain was successfully determined. The identified virus was interestingly characterized as a presumed progenitor of subtypes a and c by recombination region and phylogenetic tree analyses. In conclusion, the present infection is, to ou r knowledge, the first case of molecularly identified and genetically characterized HTLV-2 infection found via prenatal screening in nonendemic Japan.