Altered expression of mRNA profiles in blood of early-onset schizophrenia.
Altered expression of mRNA profiles in blood of early-onset schizophrenia.
复制标题
早发性精神分裂症血液中 mRNA 表达谱的改变
DOI：
10.1038/srep16767
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发表时间：
2016-01-06
影响因子：
4.6
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Xu Y;Yao Shugart Y;Wang G;Cheng Z;Jin C;Zhang K;Wang J;Yu H;Yue W;Zhang F;Zhang D
为了确定精神分裂症（SZ）的基因表达异常，我们产生了全基因组基因表达谱，使用微阵列对外周血单个核细胞（PBMC）从18个早发性SZ病例和12个对照。我们检测到84个转录差异表达的诊断状态，82个基因被上调和2下调。我们鉴定了两个SZ相关基因共表达模块（绿色和红色），包括446个基因。绿色模块与SZ正相关，主要包括SZ中上调的基因;而红色模块与疾病状态负相关，主要涉及SZ中名义上下调的基因。嗅觉传导途径是两个模块中基因最丰富的途径。利用实时定量PCR验证了包括AKT 1、BRCA 1、CCDC 134、UBD和ZIC 2在内的几个枢纽基因的表达水平。我们的研究结果表明，mRNA共表达异常可能作为一个有前途的机制，发展的SZ。
To identify gene expression abnormalities in schizophrenia (SZ), we generated whole-genome gene expression profiles using microarrays on peripheral blood mononuclear cells (PBMCs) from 18 early-onset SZ cases and 12 controls. We detected 84 transcripts differentially expressed by diagnostic status, with 82 genes being upregulated and 2 downregulated. We identified two SZ associated gene coexpression modules (green and red), including 446 genes . The green module is positively correlated with SZ, encompassing predominantly up-regulated genes in SZ; while the red module was negatively correlated with disease status, involving mostly nominally down-regulated genes in SZ. The olfactory transduction pathway was the most enriched pathways for the genes within the two modules. The expression levels of several hub genes, including AKT1, BRCA1, CCDC134, UBD and ZIC2 were validated using real-time quantitative PCR. Our findings indicate that mRNA coexpression abnormalities may serve as a promising mechanism underlying the development of SZ.