Protective effect of flavonoid extract from Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruit on alcoholic liver oxidative injury in mice
Protective effect of flavonoid extract from Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruit on alcoholic liver oxidative injury in mice
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DOI：
10.1007/s11418-014-0829-9
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发表时间：
2014-07-01
影响因子：
3.3
通讯作者：
Li, Peipei
中科院分区：
文献类型：
--
作者：
Liu, Hesheng;Qi, Xiangyang;Li, Peipei
评价杨梅（Myrica rubra Sieb.）等Zucc。）为研究黄酮提取物（CBFE）对慢性酒精性肝氧化损伤小鼠的保护作用，采用灌胃法给予不同剂量的CBFE（50-200 mg/kg），连续4周。检测血清、肝组织、线粒体和微粒体的生化标志物和抗氧化酶。结果表明，与酒精组相比，CBFE组血清TC、TG、L-DLC活性、肝微粒体CYP 2 E1活性、肝组织和线粒体MDA含量均显著降低（P < 0.05）。血清ALT、AST、H-DLC活性、肝组织和线粒体抗氧化酶GSH-Px、SOD、GST活性及肝微粒体HO-1活性均显著升高（P < 0.05）。组织学检查显示CBFE（200 mg/kg）预处理可显著防止酒精诱导的肝细胞凋亡和脂肪变性。这表明，CBFE对酒精诱导的肝氧化损伤的保护作用可能是由于其强大的抗氧化特性。
To evaluate the beneficial effects of Chinese bayberry (Myrica rubra Sieb. et Zucc.) flavonoid extract (CBFE) on chronic alcohol-induced liver oxidative injury in mice, experimental mice were pretreated with different doses of CBFE (50-200 mg/kg) for 4 weeks by gavage feeding. Biochemical markers and enzymatic antioxidants from serum, liver tissue, mitochondria and microsomes were examined. Our results showed that the activities of TC, TG, L-DLC in serum, the activity of CYP2E1 in microsomes, and the levels of MDA in liver tissue and mitochondria, decreased significantly (P < 0.05) in the CBFE-treated group compared with the alcohol group. On the contrary, the activities of ALT, AST, and H-DLC in serum, enzymatic antioxidants GSH-Px, SOD and GST in liver tissue and mitochondria, and HO-1 in microsomes rose markedly (P < 0.05). Histopathological examination revealed that CBFE (200 mg/kg) pretreatment noticeably prevented alcohol-induced hepatocyte apoptosis and fatty degeneration. It was suggested that the hepatoprotective effects exhibited by CBFE on alcohol-induced liver oxidative injury may be due to its potent antioxidant properties.