Acetogenic microbial degradation of vinyl chloride
Acetogenic microbial degradation of vinyl chloride
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DOI：
10.1021/es991371m
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发表时间：
2000-07-01
影响因子：
11.4
通讯作者：
Chapelle, FH
中科院分区：
文献类型：
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作者：
Bradley, PM;Chapelle, FH
在产甲烷条件下，[1,2- c -14]氯乙烯（VC）的微生物降解效果显著（最大回收率为14 +/- 3%），但其放射性与c -14-醋酸酯相当。随后，c -14-乙酸酯降解为(CH4)- c -14和(CO2)- c -14（最终回收率分别为18 +/- 2%和54 +/- 3%）。相比之下，在2-溴乙磺酸（BES）改性条件下，整个研究过程中c -14-乙酸酯的回收率很高（最大回收率为27 +/- 1%），没有(CH4)- c -14的产生，(CO2)- c -14的最终回收率仅为35 +/- 4%。这些结果表明，氧化产氢可能是厌氧VC生物降解的重要机制。此外，这些结果(1)表明，微生物将VC降解为CH4和CO2可能涉及氧化丙酮生成，然后是丙酮营养甲烷生成；(2)表明氧化丙酮生成可能是之前报道的在Fe（III）还原、so4还原和腐植酸还原条件下VC净氧化为CO2的第一步。
Under methanogenic conditions, microbial degradation of [1,2-C-14]vinyl chloride (VC) resulted in significant (14 +/- 3% maximum recovery) but transient rec every of radioactivity as C-14-acetate. Subsequently, C-14-acetate was degraded to (CH4)-C-14 and (CO2)-C-14 (18 +/- 2% and 54 +/- 3% final recoveries, respectively). In contrast, under 2-bromoethanesulfonic acid (BES) amended conditions, C-14-acetate recovery remained high (27 +/- 1% maximum recovery) throughout the study, no (CH4)-C-14 was produced, and the final recovery of (CO2)-C-14 was only 35 +/- 4%. These results demonstrate that oxidative acetogenesis may be an important mechanism for anaerobic VC biodegradation. Moreover, these results (1) demonstrate that microbial degradation of VC to CH4 and CO2 may involve oxidative acetogenesis followed by acetotrophic methanogenesis and (2) suggest that oxidative acetogenesis may be the initial step in the net oxidation of VC to CO2 reported previously under Fe(III)-reducing, SO4-reducing, and humic acids-reducing conditions.