Novel electrochemical aptamer biosensor based on an enzyme-gold nanoparticle dual label for the ultrasensitive detection of epithelial tumour marker MUC1
Novel electrochemical aptamer biosensor based on an enzyme-gold nanoparticle dual label for the ultrasensitive detection of epithelial tumour marker MUC1
复制标题
基于酶-金纳米粒子双标记的新型电化学适体生物传感器，用于超灵敏检测上皮肿瘤标志物MUC1
DOI：
10.1016/j.bios.2013.10.015
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发表时间：
2014-03-15
影响因子：
12.6
通讯作者：
Wang, Shengfu
中科院分区：
文献类型：
--
作者：
Hu, Rong;Wen, Wei;Wang, Shengfu
在该测定中开发了一种基于发夹寡核苷酸（HO）开关、金纳米颗粒（AuNPs）和酶信号放大的用于超灵敏检测粘蛋白1蛋白（MUC1）的新平台。将该HO适体和辣根过氧化物酶（HRP）固定在AuNP上以产生HO-AuNP-HRP缀合物。AuNPs被用作HO和HRP之间的标记和桥梁。HRP也被用作催化H2O2氧化邻苯二胺的标记物。反应产物为2，3-二氨基吩嗪（DAP），DAP被还原后可在修饰电极表面检测到。DAP的还原信号被用作灵敏检测的探针。在寡核苷酸与MUC1之间的识别之后，暴露生物素。生物素沿着偶联物被链霉亲和素捕获到修饰电极表面。因此，通过检测DAP的电化学还原信号，可以灵敏地检测粘蛋白家族的膜相关糖蛋白MUC1。与其他适体传感器相比，该生物传感器具有良好的线性范围为8.8 nM至353.3 nM，检测下限为2.2 nM的MUC1。该方法为MUC 1的检测提供了一种新的电化学方法。(C)2013爱思唯尔有限公司版权所有。
A novel platform based on a hairpin oligonucleotide (HO) switch, gold nanoparticles (AuNPs), and enzyme signal amplification for the ultrasensitive detection of mucin 1 protein (MUC1) was developed in this assay. This HO aptamers and horseradish peroxidase (HRP) were immobilised on the AuNPs to yield HO-AuNP-HRP conjugates. AuNPs were used as labels and bridges between the HO and HRP. HRP was also used as label for catalysing the oxidation of o-phenylenediamine by H2O2. The reaction product was 2,3-diaminophenazine (DAP), which was reduced and could be detected at surface of modified electrode. The reduction signal of DAP was used as a probe for the sensitive detection. After the recognition between oligonucleotide and MUC1, biotin was exposed. Biotin, along with the conjugate, was captured by streptavidin onto the surface of modified electrode. Therefore, the detection of target MUC1 which was a membrane-associated glycoprotein of the mucin family could be sensitively transduced via detection of the electrochemical reduction signal of DAP. Compared to other aptasensors, this biosensor has a good linear correlation ranges from 8.8 nM to 353.3 nM and a lower detection limit of 2.2 nM for MUC1. The proposed method provided a new electrochemical approach for the detection of MUC1. (C) 2013 Elsevier B.V. All rights reserved.