ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review.
ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review.
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作者：
Dormatey R;Sun C;Ali K;Fiaz S;Xu D;Calderón-Urrea A;Bi Z;Zhang J;Bai J
抗抗生素和抗除草剂基因是植物转化中最常见的标记基因，用于提高作物产量和食品品质。然而，公众对在粮食作物中使用抗性标记基因感到担忧，因为潜在的基因可能从转基因植物流向相容的杂草亲戚，导致“超级杂草”和抗生素耐药性的可能发展。aph、nptII、aaC3、aadA、pat、bar、epsp、gat等选择性标记基因通过遗传转化制备转基因植物，但存在一定的局限性。这些标记基因赋予抗生素或除草剂抗性，并与具有经济价值的基因一起引入作物，主要存在三个问题：选择性制剂对植物细胞增殖和分化有负面影响，许多可选择标记基因的环境影响不确定，以及难以用相同的可选择标记对金字塔所需基因进行反复转化。最近，提出了一种简单、新颖、经济的方法，通过基因表达编码亚磷酸酯氧化还原酶（PTXD）酶，将植物细胞中不可代谢的亚磷酸酯（Phi）转化为细胞发育所需的重要磷酸盐（Pi）。ptxD基因与含Phi作为唯一磷源的选择培养基结合，可以作为一种有效的转化细胞选择系统。这种选择系统为转基因植物增加了营养，对环境没有潜在风险。ptxD/Phi系统与其他基于抗生素的转基因选择系统相比，具有成本和安全性方面的优势，是一种很有前景的转基因选择系统。在这篇综述中，我们总结了遗传转化选择标记的发展和ptxD/Phi方案作为替代选择标记系统的潜在用途，以尽量减少未来抗生素和除草剂标记基因的使用。
Antibiotic and herbicide resistance genes are the most common marker genes for plant transformation to improve crop yield and food quality. However, there is public concern about the use of resistance marker genes in food crops due to the risk of potential gene flow from transgenic plants to compatible weedy relatives, leading to the possible development of “superweeds” and antibiotic resistance. Several selectable marker genes such as aph, nptII, aaC3, aadA, pat, bar, epsp and gat, which have been synthesized to generate transgenic plants by genetic transformation, have shown some limitations. These marker genes, which confer antibiotic or herbicide resistance and are introduced into crops along with economically valuable genes, have three main problems: selective agents have negative effects on plant cell proliferation and differentiation, uncertainty about the environmental effects of many selectable marker genes, and difficulty in performing recurrent transformations with the same selectable marker to pyramid desired genes. Recently, a simple, novel, and affordable method was presented for plant cells to convert non-metabolizable phosphite (Phi) to an important phosphate (Pi) for developing cells by gene expression encoding a phosphite oxidoreductase (PTXD) enzyme. The ptxD gene, in combination with a selection medium containing Phi as the sole phosphorus (P) source, can serve as an effective and efficient system for selecting transformed cells. The selection system adds nutrients to transgenic plants without potential risks to the environment. The ptxD/Phi system has been shown to be a promising transgenic selection system with several advantages in cost and safety compared to other antibiotic-based selection systems. In this review, we have summarized the development of selection markers for genetic transformation and the potential use of the ptxD/Phi scheme as an alternative selection marker system to minimize the future use of antibiotic and herbicide marker genes.
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影响因子：
6.2
作者：
Bhatnagar, Madhurima;Prasad, Kalyani;Sharma, Kiran K.
通讯作者：
Sharma, Kiran K.
影响因子：
5
作者：
Banchi, Elisa;Carniel, Fabio Candotto;Tretiach, Mauro
通讯作者：
Tretiach, Mauro
DOI：
10.1016/j.pnsc.2008.08.005
发表时间：
2009-05-10
影响因子：
4.7
作者：
Bai, Yunfeng;Guo, Zhihua;Zhang, Weifeng
通讯作者：
Zhang, Weifeng
影响因子：
2.4
作者：
Bakhsh, Allah
通讯作者：
Bakhsh, Allah
影响因子：
4.3
作者：
Brandt, P
通讯作者：
Brandt, P