Structure characterization and identification of steroidal saponins from the rhizomes of Anemarrhena asphodeloides by ultra performance liquid chromatography and hybrid quadrupole time-of-flight mass spectrometry
Structure characterization and identification of steroidal saponins from the rhizomes of Anemarrhena asphodeloides by ultra performance liquid chromatography and hybrid quadrupole time-of-flight mass spectrometry
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超高效液相色谱和混合四极杆飞行时间质谱法对知母根茎中甾体皂苷的结构表征和鉴定
DOI：
10.1016/j.ijms.2013.03.005
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发表时间：
2013-05-01
影响因子：
1.8
通讯作者：
Ma, Baiping
中科院分区：
文献类型：
--
作者：
Zhao, Yang;Kang, Liping;Ma, Baiping
采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-Q-TOF-MSE）对知母粗提物中的甾体皂苷类化合物进行分离鉴定。采用UPLC/Qtof MSE技术，研究了7种已知甾体皂苷类化合物在正负离子模式下的裂解规律。通过使用这种策略，完整的前体和碎片离子的信息，从一个单一的注射获得。根据质谱、碎片离子、峰的保留时间，并与文献中已知化合物和标准品进行比较，从紫茎泽兰中鉴定或初步鉴定出67种甾体皂苷。类曲霉。这包括16组异构体。其中14个皂苷类化合物为新化合物。本研究为利用UPLC/Qtof MSE快速鉴定中药等复杂样品中的化学成分提供了一个很好的范例，同时避免了从粗提物中分离纯化化合物的费时和繁琐。(C)2013爱思唯尔有限公司版权所有。
In this work, ultra performance liquid chromatography (UPLC) coupled with a hybrid quadrupole time-of-flight mass spectrometry (Q-TOF MSE) was used to separate and identify steroidal saponins from the crude extracts of Anemarrhena asphodeloides. The fragmentation patterns of seven known steroidal saponins, which could be classified into six types, were investigated by UPLC/Qtof MSE technique in both negative and positive ion modes. By using this strategy, both intact precursor and fragment ion information were obtained from a single injection. Based on the exact mass, fragment ions, and retention times of peaks, and comparison with reference standards and known compounds in the literature, sixty-seven steroidal saponins were identified or tentatively identified from A. asphodeloides. This included sixteen groups of isomers. Fourteen of these saponins were identified as new compounds. This study provides a good example for the rapid identification of chemical constituents in complex samples such as TCM by UPLC/Qtof MSE, in the mean time, avoiding the time-consuming and tedious purification of compounds from the crude extracts. (C) 2013 Elsevier B.V. All rights reserved.