In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering
In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering
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DOI：
10.1016/j.msec.2020.111194
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发表时间：
2020-11-01
影响因子：
7.9
通讯作者：
Miyaji, Hirofumi
中科院分区：
文献类型：
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作者：
Santhakumar, Syama;Oyane, Ayako;Miyaji, Hirofumi
无定形磷酸钙（ACP）在人体硬组织三维（3D）胶原网络中的生物矿化中起重要作用，并具有骨传导性。多孔胶原海绵包裹ACP纳米颗粒可被认为是骨组织工程的潜在支架材料。在这项研究中，这样的复合材料是通过均匀的ACP沉淀使用过饱和的磷酸钙（CaP）溶液。均匀的ACP沉淀诱导原位内的海绵由温度控制的涂层过程由两个步骤组成。在第一步中，将CaP溶液冷却至4摄氏度以抑制沉淀，直到溶液完全渗透到海绵的内部孔隙中。在第二步中，将CaP溶液加热至25 ° C，同时连续振荡以诱导海绵内的ACP沉淀。因此，所得海绵在其内表面和外表面上涂覆有ACP纳米颗粒。模拟体液（SBF）测试表明涂覆有ACP纳米颗粒的胶原海绵的骨传导性。此外，使用补充有碱性成纤维细胞生长因子（bFGF）的CaP溶液制造固定有碱性成纤维细胞生长因子（bFGF）的ACP涂覆的胶原海绵。制造的海绵允许在培养基中持续释放bFGF，并增强成骨细胞MC 3 T3-E1细胞的增殖。这种ACP包被的胶原海绵有可能被用作骨组织工程的支架，如果追求进一步的体外和体内研究。
Amorphous calcium phosphate (ACP) plays an important role in biomineralization within the three-dimensional (3D) collagen network in human hard tissues, and exhibits osteoconductivity. Porous collagen sponges coated with ACP nanoparticles could be considered as potential scaffolds for use in bone tissue engineering. In this study, such composite materials were fabricated via homogeneous ACP precipitation using a supersaturated calcium phosphate (CaP) solution. Homogeneous ACP precipitation was induced in situ within the sponges by a temperature-controlled coating process composed of two steps. In the first step, the CaP solution was cooled to 4 degrees C to suppress precipitation until the solution penetrated fully into the sponge's internal pores. In the second step, the CaP solution was warmed up to 25 degrees C with continuous shaking to induce ACP precipitation within the sponges. The resulting sponges were therefore coated with ACP nanoparticles on their inner and outer surfaces. A simulated body fluid (SBF) test indicated osteoconductivity of the collagen sponges coated with ACP nanoparticles. Further, ACP-coated collagen sponges immobilizing basic fibroblast growth factor (bFGF) were fabricated using the CaP solution supplemented with bFGF. The fabricated sponges allowed the sustained release of bFGF in a culture medium and enhanced proliferation of osteoblastic MC3T3-E1 cells. Such ACP-coated collagen sponges have the potential to be used as scaffolds in bone tissue engineering if pursued for further in vitro and in vivo studies.