Immunocytochemical localization of photopigments in cephalopod retinae.
Immunocytochemical localization of photopigments in cephalopod retinae.
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头足类视网膜感光色素的免疫细胞化学定位。
DOI：
10.1007/bf01611350
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Bok,D
中科院分区：
文献类型：
--
作者：
Robles,LJ;Watanabe,A;Kremer,NE;Wong,F;Bok,D
利用光镜和电镜免疫细胞化学方法，对头足类动物视网膜中的视紫红质和视色素进行了定位研究。针对鱿鱼视蛋白制备的多克隆抗体证明了这种蛋白质存在于章鱼视网膜的感光体横纹肌，高尔基体区，高尔基体相关的囊泡，质膜，大细胞质囊泡和轴突膜。抗视蛋白单克隆抗体对鱿鱼感光细胞内节的横纹肌和多泡体进行了免疫染色。我们认为多泡体参与了横纹肌的更新。多克隆抗视网膜色素定位这种色素的感光细胞内节的髓样体，横纹肌，和相对的横纹肌之间的细胞外空间在章鱼视网膜。结果表明，视紫红质和视黄色素之间的一些有趣的功能关系，就发色团之间的交换照明形式的这些色素和发色团除了新合成的视蛋白。
The photopigments, rhodopsin and retinochrome, have been localized in cephalopod retinae using light and electron microscopic immunocytochemical methods. Polyclonal antibodies prepared against squid opsin demonstrated the presence of this protein in the photoreceptor rhabdomes, Golgi zone, Golgi-associated vesicles, plasma membrane, large cytoplasmic vesicles, and axonal membranes of octopus retinae. Monoclonal anti-opsin immunostained the rhabdomes and multivesicular bodies in the photoreceptor inner segments of squid. We believe the multivesicular bodies are involved in rhabdome turnover. Polyclonal anti-retinochrome localized this photopigment to the myeloid bodies of the photoreceptor inner segments, the rhabdomes, and to the extracellular space between opposing rhabdomeres in octopus retina. The results suggest some interesting functional relationships between rhodopsin and retinochrome with regard to chromophore exchange between illuminated forms of these photopigments and chromophore addition to newly synthesized opsin.