A newly identified hepatitis B type virus in tree squirrels.
A newly identified hepatitis B type virus in tree squirrels.
复制标题
树松鼠体内新发现的乙型肝炎病毒。
DOI：
10.1073/pnas.83.7.2233
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发表时间：
1986
影响因子：
11.1
通讯作者：
Blumberg,BS
中科院分区：
文献类型：
--
作者：
Feitelson,MA;Millman,I;Halbherr,T;Simmons,H;Blumberg,BS
病毒相关颗粒已从三种常见的灰树松鼠（松鼠carolinensis pennsylvania）的肝脏中分离出来，这些松鼠具有肝炎的组织学证据。其中两个肝脏也通过地衣红染色呈阳性，表明肝细胞胞质中存在表面抗原。通过CsCl密度平衡梯度离心对这些颗粒进行分级，并对表面抗原、核心抗原和DNA聚合酶活性的级分进行测定，结果表明所有这三种颗粒均存在，密度峰值约为1.27。纯化病毒制剂的电子显微镜检查显示平均直径为25 nm的球形颗粒。初步表征的DNA聚合酶产物的凝胶电泳显示一个单一的DNase I敏感带，迁移略快于土拨鼠肝炎病毒DNA聚合酶产物。在固相分析中，通过纯化的乙型肝炎B表面和/或核心抗原与某些松鼠血清结合证明存在明显的交叉反应抗体。受感染的树松鼠的血清中似乎缺乏可检测到的抗原。这些结果表明，研究的树松鼠是慢性携带者的B型肝炎病毒。本文所述的宿主-病毒相互作用可用于理解与人类B型肝炎相关的慢性携带状态。
Virus-associated particles have been isolated from the livers of three common gray tree squirrels (Sciurus carolinensis pennsylvanicus) that have histological evidence of hepatitis. Two of these livers were also positive by orcein staining, suggesting the presence of surface antigen in the cytoplasm of hepatocytes. Fractionation of these particles by CsCl density equilibrium gradient centrifugation and assay of the fractions for surface antigen, core antigen, and DNA polymerase activities demonstrate the presence of all three at an approximate density peak of 1.27. Electron microscopic examination of purified virus preparations showed spherical particles with a mean diameter of 25 nm. Initial characterization of the DNA polymerase product by gel electrophoresis showed a single DNase I sensitive band, migrating slightly faster than the woodchuck hepatitis virus DNA polymerase product. The presence of apparently cross-reacting antibodies was demonstrated by purified hepatitis B surface and/or core antigens binding to some squirrel sera in solid phase assays. Infected tree squirrels appear to lack detectable antigen in their sera. These results suggest that the tree squirrels studied are chronic carriers of a hepatitis B type virus. The host-virus interaction described herein may be useful in understanding the chronic carrier state associated with hepatitis B in man.