The effect of semi-dwarf genes on root system size in field-grown barley
The effect of semi-dwarf genes on root system size in field-grown barley
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DOI：
10.1007/s00122-005-0147-4
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发表时间：
2006-03-01
影响因子：
5.4
通讯作者：
Thomas, WTB
中科院分区：
文献类型：
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作者：
Chloupek, O;Forster, BP;Thomas, WTB
用电容法测定了12个不同大麦品种和157个双单倍体大麦品系的根系大小。DHS的亲本德尔卡多和B83-12/21/5分别携带不同的半矮秆基因sdw1和ari-e.gp。在植株发育期间，田间对RSS的估计有三次：茎伸长(RSS1)、抽穗(RSS2)和籽粒灌浆(RSS3)。每年在两个或三个地点对12个大麦基因型进行评估；2002年在两个地点对卫生署定位人群进行评估。在12个大麦品种中，具有半矮秆基因的大麦在3年内的RSS值均高于非半矮秆对照(1、2、3年分别为28.9%、24.6%和15.0%)。双亲均数两侧的群体表现为超亲分离，表明RSS为多基因控制。在1H、3H、4H、5H和7H 7条染色体上检测到RSS的数量性状基因座(QTL)，并与已定位的农艺性状进行了比较。3 H上的总RSS QTL与株高、单株产量和单株重的QTL分别与sdw1和QTL连锁。5H上的RSS3 QTL与Ari-e.GP和Ari-e.GP连锁，控制株高、产量、株重、收获指数和分蘖数。7H上的RSS3 QTL还与总RSS QTL和株高、收获指数QTL相关联。其他RSS QTL与所研究的任何其他性状均不相关。RSS被认为是一个与重要性状，特别是产量相关的多基因性状。这项研究强调了半矮化基因的影响，并讨论了根部性状育种的潜力。
Root system size (RSS) was measured in 12 diverse barley genotypes and 157 double-haploid lines (DHs), using electric capacitance. The parents of the DHs, Derkado and B83-12/21/5, carry different semi-dwarfing genes, sdw1 and ari-e.GP, respectively. Estimates of RSS were taken in the field thrice during plant development: stem elongation (RSS1), heading (RSS2) and grain filling (RSS3). The 12 barley genotypes were assessed over 3 years and at two or three locations each year; the DH mapping population was assessed at two locations in 2002. Among the 12 barley genotypes, those with the semi-dwarf genes had greater RSS values in all 3 years (28.9, 24.6 and 15.0% in years 1, 2 and 3, respectively) compared to non-semi-dwarf controls. The DH population showed transgressive segregation on both sides of the parent means, indicating polygenic control of RSS. Quantitative trait loci (QTLs) for RSS were found on five of the seven chromosomes: 1H, 3H, 4H, 5H and 7H and these were compared with previously mapped agronomic traits. The TotalRSS QTL on 3H was associated with sdw1 and QTLs for height, plant yield and plant weight. The RSS3 QTL on 5H was associated with ari-e.GP and QTLs for height, plant yield, plant weight, harvest index and tiller number. The RSS3 QTL on 7H was also associated with a TotalRSS QTL and QTLs for plant weight and harvest index. Other RSS QTLs were not associated with any other trait studied. RSS is considered to be a polygenic trait linked to important traits, in particular to yield. The study highlights the effects of semi-dwarfing genes and discusses the potential for breeding for root traits.