Immunological Effect of Active Hexose Correlated Compound (AHCC) in Healthy Volunteers: A Double-Blind, Placebo-Controlled Trial
Immunological Effect of Active Hexose Correlated Compound (AHCC) in Healthy Volunteers: A Double-Blind, Placebo-Controlled Trial
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DOI：
10.1080/01635580801993280
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发表时间：
2008-09-01
影响因子：
2.9
通讯作者：
Kamiyama, Yasuo
中科院分区：
文献类型：
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作者：
Terakawa, Naoyoshi;Matsui, Yoichi;Kamiyama, Yasuo
本研究旨在通过检测健康志愿者外周血中树突状细胞(DC)的数量和功能来评价摄入活性己糖相关化合物(AHCC)对免疫应答的影响。21名健康志愿者被随机分成两组，分别服用安慰剂或AHCC，剂量为3.0g/d，共4wk。检测外周血中CD11c(+)、DC(Dc1)和CD11c(-)DC(Dc2)的数量。进行异基因混合白细胞反应(MLR)。测定自然杀伤(NK)细胞活性和T淋巴细胞对有丝分裂原(植物血凝素[PHA])的增殖反应。检测内毒素[IL-2、IL-4、IL-6、IL-10、干扰素-γ、肿瘤坏死因子-α]刺激的细胞因子的产生。与基线和对照组(n=11)相比，AHCC组(n=10)的DC总数显著增加。AHCC组患者入院后DC 1数量显著高于基线水平。AHCC组DC_2较对照组显著增加。AHCC组的MLR明显高于对照组。两组间在PHA、NK细胞活性和细胞因子产生方面无显著差异。AHCC摄入可导致DC数量增加，DC功能增强，参与特异性免疫。
The aim of this study was to evaluate the effects of active hexose correlated compound (AHCC) intake on immune responses by investigating the number and function of circulating dendritic cells (DCs) in healthy volunteers. Twenty-one healthy volunteers were randomized to receive placebo or AHCC at 3.0 g/day for 4 wk. The number of circulating cluster of differentiation (CD)11c(+) DCs (DC1) and CD11c(-) DCs (DC2) were measured. Allogeneic mixed-leukocyte reaction (MLR) was performed. Natural killer (NK) cell activity and the proliferative response of T lymphocytes toward mitogen (phytohemagglutinin [PHA]) were measured. We also measured cytokine production stimulated by lipopolysaccharide [interleukin (IL)-2, IL-4, IL-6, IL-10, interferon gamma-gamma, tumor necrosis factor-alpha). The AHCC group (n = 10) after AHCC intake had a significantly higher number of total DCs compared to that at baseline and values from control subjects (n = 11). The number of DC1s in the AHCC group after intake was significantly higher than at baseline. DC2s in the AHCC group were significantly increased in comparison with controls. The MLR in the AHCC group was significantly increased compared to controls. No significant differences in PHA, NK cell activity, and cytokine production were found between groups. AHCC intake resulted in the increased number of DCs and function of DC1s, which have a role in specific immunity.