Rapid separation of high-mannose-type oligosaccharides by high-pressure liquid chromatography.
Rapid separation of high-mannose-type oligosaccharides by high-pressure liquid chromatography.
复制标题
高压液相色谱法快速分离高甘露糖型低聚糖。
DOI：
10.1016/0003-2697(81)90582-0
复制
发表时间：
1981
影响因子：
2.9
通讯作者：
Turco,SJ
中科院分区：
文献类型：
--
作者：
Turco,SJ
在有机胺修饰的硅胶上，用液相色谱分离了由多醇连接的低聚糖中间体衍生的中性低聚糖。以乙腈和水为流动相时，这些低聚糖在含化学键合氨丙基的硅胶或含0.05%1，4-二氨基丁烷的硅胶上的拆分时间不到1.5h。
Neutral oligosaccharides derived from dolichol-linked oligosaccharide intermediates have been separated by liquid chromatography on silica gel modified by organic amines. By using a mobile phase consisting of mixtures of acetonitrile and water, effective resolution of these oligosaccharides was achieved in less than 1.5 h on silica bearing chemically bonded aminopropyl groups or on silica with 0.05% 1,4-diaminobutane present in the mobile phase.